National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, P.R. China.
Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing 100083, P.R. China.
Plant Physiol. 2023 Mar 17;191(3):1702-1718. doi: 10.1093/plphys/kiac585.
Leaf margins are complex plant morphological features that contribute to leaf shape diversity, which affects plant structure, yield, and adaptation. Although several leaf margin regulators have been identified to date, the genetic basis of their natural variation has not been fully elucidated. In this study, we profiled two distinct leaf morphology types (serrated and smooth) using the persistent homology mathematical framework (PHMF) in two poplar species (Populus tomentosa and Populus simonii, respectively). A combined genome-wide association study (GWAS) and expression quantitative trait nucleotide (eQTN) mapping were applied to create a leaf morphology control module using data from P. tomentosa and P. simonii populations. Natural variation in leaf margins was associated with YABBY11 (YAB11) transcript abundance in poplar. In P. tomentosa, PtoYAB11 carries a premature stop codon (PtoYAB11PSC), resulting in the loss of its positive regulation of NGATHA-LIKE1 (PtoNGAL-1) and RIBULOSE BISPHOSPHATE CARBOXYLASE LARGE SUBUNIT (PtoRBCL). Overexpression of PtoYAB11PSC promoted serrated leaf margins, enlarged leaves, enhanced photosynthesis, and increased biomass. Overexpression of PsiYAB11 in P. tomentosa promoted smooth leaf margins, higher stomatal density, and greater light damage repair ability. In poplar, YAB11-NGAL1 is sensitive to environmental conditions, acts as a positive regulator of leaf margin serration, and may also link environmental signaling to leaf morphological plasticity.
叶片边缘是复杂的植物形态特征，有助于叶片形状的多样性，进而影响植物的结构、产量和适应性。尽管迄今为止已经鉴定出几种叶片边缘调控因子，但它们自然变异的遗传基础尚未完全阐明。在这项研究中，我们使用持久同调数学框架（PHMF）分别在两个杨树物种（毛白杨和银白杨）中对两种截然不同的叶片形态类型（锯齿状和光滑状）进行了分析。我们将毛白杨和银白杨种群的数据应用于全基因组关联研究（GWAS）和表达数量性状核苷酸（eQTN）图谱，以创建一个叶片形态控制模块。叶片边缘的自然变异与杨树中的 YABBY11（YAB11）转录丰度有关。在毛白杨中，PtoYAB11 携带一个提前终止密码子（PtoYAB11PSC），导致其对 NGATHA-LIKE1（PtoNGAL-1）和核酮糖二磷酸羧化酶大亚基（PtoRBCL）的正向调控作用丧失。PtoYAB11PSC 的过表达促进了锯齿状叶片边缘、叶片增大、增强光合作用和增加生物量。PsiYAB11 在毛白杨中的过表达促进了光滑的叶片边缘、更高的气孔密度和更大的光损伤修复能力。在杨树中，YAB11-NGAL1 对环境条件敏感，作为叶片边缘锯齿状的正向调控因子，并且可能将环境信号与叶片形态可塑性联系起来。
