比较基因组分析表明，柠檬酸杆菌 XT1-2-2 揭示了微生物固定重金属的分子机制。
Comparative genomic analysis of Citrobacter sp. XT1-2-2 reveals insights into the molecular mechanism of microbial immobilization of heavy metals.
机构信息
Hunan Institute of Microbiology, 410009, Changsha, Hunan, China.
Hunan Engineering and Technology Research Center of Agricultural Microbiology Application, 410009, Changsha, Hunan, China.
出版信息
BMC Genomics. 2022 Dec 19;23(1):838. doi: 10.1186/s12864-022-09069-4.
BACKGROUND
In our previous study, Citrobacter sp. XT1-2-2 was isolated from high cadmium-contaminated soils, and demonstrated an excellent ability to decrease the bioavailability of cadmium in the soil and inhibit cadmium uptake in rice. In addition, the strain XT1-2-2 could significantly promote rice growth and increase rice biomass. Therefore, the strain XT1-2-2 shows great potential for remediation of cadmium -contaminated soils. However, the genome sequence of this organism has not been reported so far. RESULTS: Here the basic characteristics and genetic diversity of the strain XT1-2-2 were described, together with the draft genome and comparative genomic results. The strain XT1-2-2 is 5040459 bp long with an average G + C content of 52.09%, and contains a total of 4801 genes. Putative genomic islands were predicted in the genome of Citrobacter sp. XT1-2-2. All genes of a complete set of sulfate reduction pathway and various putative heavy metal resistance genes in the genome were identified and analyzed.
CONCLUSIONS
These analytical results provide insights into the genomic basis of microbial immobilization of heavy metals.
背景
在我们之前的研究中，从高镉污染土壤中分离出了柠檬酸杆菌 XT1-2-2，该菌株表现出降低土壤中镉生物有效性和抑制水稻吸收镉的优异能力。此外，该菌株 XT1-2-2 还能显著促进水稻生长，增加水稻生物量。因此，该菌株 XT1-2-2 对修复镉污染土壤具有很大的潜力。然而，到目前为止，该生物的基因组序列尚未报道。
结果
本文描述了菌株 XT1-2-2 的基本特征和遗传多样性，以及基因组草图和比较基因组学的结果。菌株 XT1-2-2 全长 5040459bp，平均 G+C 含量为 52.09%，共包含 4801 个基因。预测到了柠檬酸杆菌 XT1-2-2 基因组中的假定基因组岛。鉴定和分析了基因组中硫酸盐还原途径的完整一套基因和各种假定的重金属抗性基因。
结论
这些分析结果为微生物固定重金属的基因组基础提供了新的见解。
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