Whole-Genome Analysis of CC-PT4 against MRSA, a Non- Yeast Isolated from Grape.

is often isolated from environments associated with grape and winemaking. has many beneficial properties, including the ability to improve the flavor of wine, the production of beneficial metabolites, and the ability to biocontrol. CC-PT4 (CGMCC No. 23573) was isolated from grape and can inhibit methicillin-resistant and adaptability to harsh environments. In this paper, the whole genome of CC-PT4 was sequenced and bioinformatics analyses were performed. The CC-PT4 genome was finally assembled into five scaffolds with a genome size of 9.45 Mb and a GC content of 39.5%. It was predicted that the strain contained 4150 protein-coding genes, of which two genes encoded killer toxin and one gene encoded lysostaphin. It also contains genes encoding F1F0-ATPases, Na(+)/H(+) antiporter, cation/H(+) antiporter, ATP-dependent bile acid permease, major facilitator superfamily (MFS) antiporters, and stress response protein, which help CC-PT4 adapt to bile, acid, and other stressful environments. Proteins related to flocculation and adhesion have also been identified in the CC-PT4 genome. Predicted by antiSMASH, two secondary metabolite biosynthesis gene clusters were found, and the synthesized metabolites may have antimicrobial effects. Furthermore, CC-PT4 carried genes associated with pathogenicity and drug resistance. Overall, the whole genome sequencing and analysis of CC-PT4 in this study provide valuable information for understanding the biological characteristics and further development of this strain.