Lacotte Pierre-Alexandre, Simons Alexis, Bouttier Sylvie, Malet-Villemagne Jeanne, Nicolas Valérie, Janoir Claire
INRAE, Université Paris-Saclay, AgroParisTech, Micalis Institute, 92290 Châtenay-Malabry, France.
Laboratoire Eau, Environnement et Systèmes Urbains (Leesu), Université Paris-Est Créteil, École des Ponts ParisTech, 94010 Créteil, France.
Microorganisms. 2022 May 24;10(6):1082. doi: 10.3390/microorganisms10061082.
is responsible for post-antibiotic diarrhea and most of the pseudomembranous colitis cases. Multiple recurrences, one of the major challenges faced in infection (CDI) management, can be considered as chronic infections, and the role of biofilm formation in CDI recurrences is now widely considered. Therefore, we explored if the probiotic yeast CNCM I-745 could impact the in vitro formation of biofilm. Biomass staining and viable bacterial cell quantification showed that live exerts an antagonistic effect on the biofilm formation for the three strains tested. Confocal laser scanning microscopy observation revealed a weakening and an average thickness reduction of the biofilm structure when is co-incubated with , compared to the single-species bacterial biofilm structure. These effects, that were not detected with another genetically close yeast, , seemed to require direct contact between the probiotic yeast and the bacterium. Quantification of the extrapolymeric matrix components, as well as results obtained after DNase treatment, revealed a significant decrease of eDNA, an essential structural component of the biofilm matrix, in the dual-species biofilm. This modification could explain the reduced cohesion and robustness of biofilms formed in the presence of CNCM I-745 and be involved in clinical preventive effect against CDI recurrences.
它是抗生素相关性腹泻和大多数伪膜性结肠炎病例的病因。多次复发是艰难梭菌感染（CDI）管理面临的主要挑战之一，可被视为慢性感染，生物膜形成在CDI复发中的作用现在已得到广泛认可。因此，我们探究了益生菌酵母CNCM I-745是否会影响生物膜的体外形成。生物量染色和活菌细胞定量分析表明，活菌对所测试的三种菌株的生物膜形成具有拮抗作用。共聚焦激光扫描显微镜观察显示，与单菌种细菌生物膜结构相比，当与[具体菌株]共同孵育时，生物膜结构减弱且平均厚度减小。这些效应在另一种亲缘关系较近的酵母[具体酵母]中未检测到，似乎需要益生菌酵母与细菌直接接触。对胞外聚合物基质成分的定量分析以及DNase处理后获得的结果表明，在双菌种生物膜中，胞外DNA（生物膜基质的一种重要结构成分）显著减少。这种改变可以解释在存在CNCM I-745的情况下形成的生物膜凝聚力和坚固性降低的原因，并可能参与对CDI复发的临床预防作用。
