高真核起始因子 5A2 的表达预示着不良预后，可能参与了头颈部鳞状细胞癌中的 SNHG16/miR-10b-5p/EIF5A2 调控轴。

High eukaryotic initiation factor 5A2 expression predicts poor prognosis and may participate in the SNHG16/miR-10b-5p/EIF5A2 regulatory axis in head and neck squamous cell carcinoma.

机构信息

Department of Radiation Oncology, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, China.

Department of Medical Imaging, The Affiliated Lihuili Hospital, Ningbo University, Ningbo, China.

出版信息

J Clin Lab Anal. 2023 Jan;37(1):e24820. doi: 10.1002/jcla.24820. Epub 2022 Dec 22.

DOI:10.1002/jcla.24820

PMID:36550070

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9833988/

Abstract

BACKGROUND

This study attempted to investigate the significance of eukaryotic initiation factor 5A2 (EIF5A2) in the prognosis and regulatory network of head and neck squamous cell carcinoma (HNSCC).

METHODS

EIF5A2 expression, prognostic information, and methylation levels of HNSCC were collected from the Cancer Genome Atlas (TCGA) database. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot analyses were performed to determine EIF5A2 levels in HNSCC and normal tissue samples. R software was employed for expression analysis and prognosis assessment of EIF5A2 in HNSCC. A competing endogenous RNA (ceRNA) network was generated with the starBase database. Gene set enrichment analysis (GSEA) was used to determine the enriched physiological functions and network related to high expression of EIF5A2 in HNSCC. Immune infiltration-related outcomes were acquired from the CIBERSORT and Tumor Immune Estimation Resource (TIMER) database.

RESULTS

EIF5A2 overexpression was observed in HNSCC and linked to poor progression-free survival and overall survival time. Cox regression analyses showed that EIF5A2 level was a stand-alone indicator of HNSCC patients' prognosis. A ceRNA network analysis highlighted the SNHG16/miR-10b-5p/EIF5A2 axis in EIF5A2 regulation. The GSEA results indicated that EIF5A2 was involved in complex signaling pathways. The CIBERSORT and TIMER databases revealed significant associations between EIF5A2 expression and immune cell infiltration.

CONCLUSION

EIF5A2 overexpression may be a risk factor for prognosis in HNSCC and may be regulated by the SNHG16/miR-10b-5p/EIF5A2 axis.

摘要

背景

本研究旨在探讨真核起始因子 5A2（EIF5A2）在头颈部鳞状细胞癌（HNSCC）中的预后意义和调控网络。

方法

从癌症基因组图谱（TCGA）数据库中收集 EIF5A2 表达、HNSCC 预后信息和甲基化水平。采用实时定量逆转录聚合酶链反应（qRT-PCR）和 Western blot 分析检测 HNSCC 及正常组织样本中的 EIF5A2 水平。使用 R 软件进行 EIF5A2 在 HNSCC 中的表达分析和预后评估。利用 starBase 数据库构建竞争性内源性 RNA（ceRNA）网络。采用基因集富集分析（GSEA）确定与 EIF5A2 在 HNSCC 中高表达相关的富集生理功能和网络。从 CIBERSORT 和肿瘤免疫估计资源（TIMER）数据库中获取免疫浸润相关结果。

结果

EIF5A2 在 HNSCC 中过表达，与无进展生存期和总生存期较短相关。Cox 回归分析显示，EIF5A2 水平是 HNSCC 患者独立预后指标。ceRNA 网络分析突出了 SNHG16/miR-10b-5p/EIF5A2 轴在 EIF5A2 调控中的作用。GSEA 结果表明，EIF5A2 参与了复杂的信号通路。CIBERSORT 和 TIMER 数据库显示 EIF5A2 表达与免疫细胞浸润之间存在显著关联。