Department of Otolaryngology, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Department of Otolaryngology, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, Hebei, China.
Biochem Genet. 2024 Jun;62(3):1603-1616. doi: 10.1007/s10528-023-10489-4. Epub 2023 Sep 1.
A growing body of evidence suggests that miR-5189-3p plays a critical role in multiple diseases. This study aimed to investigate the function of miR-5189-3p in laryngeal squamous cell carcinoma (LSCC) and explore its underlying mechanisms. qRT-PCR was designed to determine the expression levels of miR-5189-3p and eukaryotic translation initiation factor 5A2 (EIF5A2), while CCK-8 assay was performed to measure the effects of miR-5189-3p on cell proliferation. Transwell assay was performed to evaluate cell invasion as well as migration, and wound healing assay was applied to demonstrate cell migratory ability. Target gene prediction and luciferase reporter assay were developed to screen the possible target gene of miR-5189-3p, and Western blot was designed to measure EIF5A2 protein expression. MiR-5189-3p was down-regulated in LSCC tissues and cell lines. Up-regulation of miR-5189-3p notably inhibited cell proliferation, invasion, and migration in HEP2 and FADU cells. EIF5A2 was the potential downstream gene of miR-5189-3p, and overexpression of miR-5189-3p apparently reduced EIF5A2 expression. Moreover, reintroduction of EIF5A2 rescued the tumor suppressive effects of miR-5189-3p. MiR-5189-3p functions as a tumor inhibitor in LSCC progression via directly regulating EIF5A2 and may be a potential therapeutic target for LSCC.
越来越多的证据表明，miR-5189-3p 在多种疾病中发挥着关键作用。本研究旨在探讨 miR-5189-3p 在喉鳞状细胞癌（LSCC）中的功能及其潜在机制。通过 qRT-PCR 确定 miR-5189-3p 和真核翻译起始因子 5A2（EIF5A2）的表达水平，通过 CCK-8 测定评估 miR-5189-3p 对细胞增殖的影响。通过 Transwell 测定评估细胞侵袭和迁移，通过划痕愈合实验评估细胞迁移能力。通过靶基因预测和荧光素酶报告基因测定筛选 miR-5189-3p 的可能靶基因，并通过 Western blot 测定 EIF5A2 蛋白表达。miR-5189-3p 在 LSCC 组织和细胞系中下调。miR-5189-3p 的上调显著抑制 HEP2 和 FADU 细胞的增殖、侵袭和迁移。EIF5A2 是 miR-5189-3p 的潜在下游基因，miR-5189-3p 的过表达明显降低 EIF5A2 的表达。此外，EIF5A2 的再引入挽救了 miR-5189-3p 的肿瘤抑制作用。miR-5189-3p 通过直接调节 EIF5A2 在 LSCC 进展中发挥肿瘤抑制作用，可能是 LSCC 的潜在治疗靶点。
