College of Chemical and Pharmaceutical Engineering, Henan University of Science and Technology, Luoyang, 471023, People's Republic of China.
Biotechnol Lett. 2023 Feb;45(2):255-262. doi: 10.1007/s10529-022-03338-8. Epub 2022 Dec 22.
S-Adenosylmethionine (SAM) is a crucial small-molecule metabolite widely used in food and medicine. The development of high-throughput biosensors for SAM biosynthesis can significantly improve the titer of SAM. This paper constructed a synthetic transcription factor (TF)-based biosensor for SAM detecting in Saccharomyces cerevisiae. The synthetic TF, named MetJ-hER-VP16, consists of an Escherichia coli-derived DNA-binding domain MetJ, GS linker, the human estrogen receptor binding domain hER, and the viral activation domain VP16. The synthetic biosensor is capable of sensing SAM in a dose-dependent manner with fluorescence as the output. Additionally, it is tightly regulated by the inducer SAM and β-estradiol, which means that the fluorescence output is only available when both are present together. The synthetic SAM biosensor could potentially be applied for high-throughput metabolic engineering and is expected to improve SAM production.
S-腺苷甲硫氨酸（SAM）是一种重要的小分子代谢物，广泛应用于食品和医药领域。开发高通量生物传感器用于 SAM 生物合成可以显著提高 SAM 的产量。本文构建了一种基于合成转录因子（TF）的用于检测酿酒酵母中 SAM 的生物传感器。该合成 TF 命名为 MetJ-hER-VP16，由大肠杆菌来源的 DNA 结合域 MetJ、GS 接头、人雌激素受体结合域 hER 和病毒激活域 VP16 组成。该合成生物传感器能够以荧光为输出，以剂量依赖的方式感应 SAM。此外，它受到 SAM 和 β-雌二醇的紧密调控，这意味着只有当两者同时存在时才会有荧光输出。该合成 SAM 生物传感器可用于高通量代谢工程，并有望提高 SAM 的产量。
