Muraviov Alexander N, Vinogradova Tatiana I, Remezova Anna N, Ariel Boris M, Gorelova Anna A, Orlova Nadezhda V, Yudintceva Natalia M, Esmedliaeva Diljara S, Dyakova Marina E, Dogonadze Marine Z, Zabolotnykh Natalia V, Garapach Irina A, Maslak Olga S, Kirillov Yuri A, Timofeev Sergei E, Krylova Yulia S, Yablonskiy Petr K
Saint-Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation, 191036 Saint-Petersburg, Russia.
Institute of Cytology of Russian Academy of Sciences, Tikhoretsky 4, 194064 Saint-Petersburg, Russia.
Biomedicines. 2022 Nov 28;10(12):3062. doi: 10.3390/biomedicines10123062.
In recent years, the application of mesenchymal stem cells (MSCs) has been recognized as a promising method for treatment of different diseases associated with inflammation and sclerosis, which include nephrotuberculosis. The aim of our study is to investigate the effectiveness of MSCs in the complex therapy of experimental rabbit kidney tuberculosis and to evaluate the effect of cell therapy on the reparative processes. Methods: To simulate kidney tuberculosis, a suspension of the standard strain Mycobacterium tuberculosis H37Rv (10 CFU) was used, which was injected into the cortical layer of the lower pole parenchyma of the left kidney under ultrasound control in rabbits. Anti-tuberculosis therapy (aTBT) was started on the 18th day after infection. MSCs (5 × 10 cells) were transplanted intravenously after the start of aTBT. Results: 2.5 months after infection, all animals showed renal failure. Conducted aTBT significantly reduced the level of albumin, ceruloplasmin, elastase and the severity of disorders in the proteinase/inhibitor system and increased the productive nature of inflammation. A month after MSC transplantation, the level of inflammatory reaction activity proteins decreased, the area of specific and destructive inflammation in kidneys decreased and the formation of mature connective tissue was noted, which indicates the reparative reaction activation.
近年来，间充质干细胞（MSCs）的应用已被公认为是治疗包括肾结核在内的与炎症和硬化相关的不同疾病的一种有前景的方法。我们研究的目的是探讨间充质干细胞在实验性兔肾结核综合治疗中的有效性，并评估细胞治疗对修复过程的影响。方法：为模拟肾结核，使用结核分枝杆菌H37Rv标准菌株的悬液（10 CFU），在超声引导下将其注入兔左肾下极实质的皮质层。感染后第18天开始抗结核治疗（aTBT）。在aTBT开始后静脉内移植间充质干细胞（5×10个细胞）。结果：感染后2.5个月，所有动物均出现肾衰竭。进行aTBT可显著降低白蛋白、铜蓝蛋白、弹性蛋白酶水平以及蛋白酶/抑制剂系统紊乱的严重程度，并增加炎症的产生性质。间充质干细胞移植1个月后，炎症反应活性蛋白水平降低，肾脏中特异性和破坏性炎症区域减小，并观察到成熟结缔组织的形成，这表明修复反应被激活。
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