Wang Zhaoyang, Li Ziyi, Lei Yaling, Liu Yuan, Feng Yuqing, Chen Derong, Ma Siying, Xiao Ziyan, Hu Meirong, Deng Jingxian, Wang Yuxin, Zhang Qihao, Huang Yadong, Yang Yan
Department of Cell Biology, Jinan University, Guangzhou 510632, China.
TYRAN Cosmetics Innovation Research Institute, Jinan University, Guangzhou 511447, China.
Antioxidants (Basel). 2022 Nov 22;11(12):2312. doi: 10.3390/antiox11122312.
Cyclobutane pyrimidine dimers (CPDs) are the main mutagenic DNA photoproducts caused by ultraviolet B (UVB) radiation and represent the major cause of photoaging and skin carcinogenesis. CPD photolyase can efficiently and rapidly repair CPD products. Therefore, they are candidates for the prevention of photodamage. However, these photolyases are not present in placental mammals. In this study, we produced a recombinant photolyase-thymine (rPHO) from (). The rPHO displayed CPD photorepair activity. It prevented UVB-induced DNA damage by repairing CPD photoproducts to pyrimidine monomers. Furthermore, it inhibited UVB-induced ROS production, lipid peroxidation, inflammatory responses, and apoptosis. UVB-induced wrinkle formation, epidermal hyperplasia, and collagen degradation in mice skin was significantly inhibited when the photolyase was applied topically to the skin. These results demonstrated that rPHO has promising protective effects against UVB-induced photodamage and may contribute to the development of anti-UVB skin photodamage drugs and cosmetic products.
环丁烷嘧啶二聚体（CPDs）是由紫外线B（UVB）辐射引起的主要诱变DNA光产物，是光老化和皮肤癌发生的主要原因。CPD光解酶能高效快速地修复CPD产物。因此，它们是预防光损伤的候选物质。然而，这些光解酶在胎盘哺乳动物中并不存在。在本研究中，我们从（此处原文缺失相关内容）制备了一种重组光解酶-胸腺嘧啶（rPHO）。rPHO表现出CPD光修复活性。它通过将CPD光产物修复为嘧啶单体来防止UVB诱导的DNA损伤。此外，它还抑制UVB诱导的活性氧生成、脂质过氧化、炎症反应和细胞凋亡。当将光解酶局部应用于小鼠皮肤时，UVB诱导的小鼠皮肤皱纹形成、表皮增生和胶原蛋白降解受到显著抑制。这些结果表明，rPHO对UVB诱导的光损伤具有良好的保护作用，可能有助于抗UVB皮肤光损伤药物和化妆品的开发。
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