Frontier Institute of Science and Technology and State Key Laboratory for Mechanical Behavior of Materials , Xi'an Jiaotong University , Xi'an 710054 , China.
Xi'an No. 4 Hospital, Guangren Hospital Affiliated to School of Medicine , Xi'an Jiaotong University , Xi'an 710004 , China.
Nano Lett. 2019 Nov 13;19(11):8270-8277. doi: 10.1021/acs.nanolett.9b03836. Epub 2019 Nov 1.
Nanomaterials with intrinsic enzyme-like characteristics exhibit their great potentials as alternatives to natural enzymes. Among various enzymes, the finding of substitutes of DNA photolyases, a family of photoenzymes for repairing the ultraviolet (UV)-induced DNA damage by forming cyclobutane pyrimidine dimers (CPDs) between two adjacent thymines in a DNA strand, is still unsuccessful. CPDs raise significant health concerns in various skin diseases. Essentially, DNA photolyases selectively split dimers into monomers by photoelectrons under visible-light irradiation, and this is a photocatalytic process. However, the majority of semiconductors are unprosperous due to the accompanied photogenerated reactive oxygen species (ROS), which decompose CPDs into fragments and thereby lead to a nonselective photocatalysis. Fortunately, CeO as a semiconductor might deliver the selectively photocatalytic repair of UV-induced DNA damages, where the photoelectrons are used for the CPD cleavage, and the photogenerated ROS are locally suppressed for its antioxidant nature. Herein, we reported the defective porous CeO delivered the photolyase-like activity by enhancing visible-light absorption, enabling the effective interaction between CPDs and catalysts, and subsequently triggering the selective photocleavage of CPDs into monomers. Further, cellular and animal evaluations illustrated its high potentials as alternatives to DNA photolyases.
具有内在酶样特性的纳米材料表现出替代天然酶的巨大潜力。在各种酶中，寻找 DNA 光解酶的替代品的工作仍未成功，DNA 光解酶是一类光酶，可通过在 DNA 链上两个相邻胸腺嘧啶之间形成环丁烷嘧啶二聚体 (CPD) 来修复紫外线 (UV) 诱导的 DNA 损伤。CPD 会引起各种皮肤病的严重健康问题。本质上，DNA 光解酶通过可见光照射下的光电电子选择性地将二聚体分裂成单体，这是一个光催化过程。然而，由于伴随的光生活性氧物种 (ROS)，大多数半导体都不成功，ROS 会将 CPD 分解成碎片，从而导致非选择性光催化。幸运的是，CeO 作为半导体可能会提供对 UV 诱导的 DNA 损伤的选择性光催化修复，其中光电电子用于 CPD 裂解，并且由于其抗氧化性质，光生 ROS 被局部抑制。在此，我们报道了具有缺陷多孔 CeO 的光解酶样活性通过增强可见光吸收来实现，从而实现 CPD 与催化剂之间的有效相互作用，并随后触发 CPD 选择性光裂解释放单体。此外，细胞和动物评估表明，它作为 DNA 光解酶的替代品具有很高的潜力。
