Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, National Engineering Laboratory of Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Genes (Basel). 2022 Dec 7;13(12):2308. doi: 10.3390/genes13122308.
Our previous studies found that bta-miR-106b and its corresponding target gene, , were differentially expressed between the mammary epithelium of lactating Holstein cows with extremely high and low milk protein and fat percentage, implying the potential role of bta-miR-106b in milk composition synthesis. In this study, with luciferase assay experiment, bta-miR-106b was validated to target the 3'-untranslated region (UTR) of bovine , thereby regulating its expression. Moreover, in bovine mammary epithelial cells (BMECs), over-expression of bta-miR-106b significantly down-regulated the expression at both mRNA and protein levels, and inhibitors of bta-miR-106b increased expression. Of note, we observed that bta-miR-106b accelerated cell proliferation and cell cycle, and changed the expressions of protein synthesis related pathways such as JAK-STAT and PI3K/AKT/mTOR through regulating expression. Our findings highlight the important regulatory role of bta-miR-106b in milk protein synthesis by targeting in dairy cattle.
我们之前的研究发现，bta-miR-106b 及其相应的靶基因 在泌乳荷斯坦奶牛的乳腺上皮组织中存在差异表达，这些奶牛的乳蛋白和乳脂率极高和极低，这表明 bta-miR-106b 可能在乳成分合成中发挥作用。在这项研究中，通过荧光素酶检测实验，验证了 bta-miR-106b 靶向牛 的 3'非翻译区（UTR），从而调节其表达。此外，在牛乳腺上皮细胞（BMECs）中，bta-miR-106b 的过表达显著下调 基因在 mRNA 和蛋白水平的表达，而 bta-miR-106b 的抑制剂则增加 基因的表达。值得注意的是，我们观察到 bta-miR-106b 通过调节 基因的表达，加速细胞增殖和细胞周期，并改变与蛋白质合成相关的途径如 JAK-STAT 和 PI3K/AKT/mTOR 的表达。我们的研究结果强调了 bta-miR-106b 通过靶向 在奶牛中对乳蛋白合成的重要调节作用。
