Biological Control Research Institute, Fujian Agriculture and Forestry University, China Fruit Fly Research and Control Center of FAO/IAEA, Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou 350002, China.
Genes (Basel). 2022 Dec 11;13(12):2340. doi: 10.3390/genes13122340.
As next-generation sequencing technology becomes more mature and the cost of sequencing continues to fall, researchers are increasingly using mitochondrial genomes to explore phylogenetic relationships among different groups. In this study, we sequenced and analyzed the complete mitochondrial genomes of and sp. We predicted the secondary-structure tRNA genes of these two species and found that 21 of the 22 tRNA genes in sp. exhibited typical clover-leaf structures, with being the lone exception. In , we found that, in addition to , the secondary structure of was also incomplete, with only DHU arms and anticodon loop remaining. In addition, we found that compositional heterogeneity and variable rates of evolution are prevalent in Chalcidoidea. Under the homogeneity model, a Eupelmidae + Encyrtidae sister group relationship was proposed. Different datasets based on the heterogeneity model produced different tree topologies, but all tree topologies contained Chalcididae and Trichogrammatidae in the basal position of the tree. This is the first study to consider the phylogenetic relationships of Chalcidoidea by comparing a heterogeneity model with a homogeneity model.
随着新一代测序技术的不断成熟和测序成本的持续降低，研究人员越来越多地利用线粒体基因组来探索不同群体之间的系统发育关系。在这项研究中，我们对 和 sp. 的完整线粒体基因组进行了测序和分析。我们预测了这两个物种的二级结构 tRNA 基因，并发现 sp. 中的 21 个 tRNA 基因呈现典型的三叶草结构，其中 是唯一的例外。在 中，我们发现，除了 之外， 的二级结构也不完整，只有 DHU 臂和反密码环保留。此外，我们发现膜翅目目内存在组成异质性和进化速率变化。在均一性模型下，提出了一个 Eupelmidae + Encyrtidae 姐妹群关系。基于异质性模型的不同数据集产生了不同的树拓扑结构，但所有树拓扑结构都在树的基部包含了小蜂科和茧蜂科。这是首次通过比较异质性模型和均一性模型来考虑膜翅目目内的系统发育关系。
