Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture animals, Shanghai 201306, China.
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Genes (Basel). 2022 Dec 16;13(12):2384. doi: 10.3390/genes13122384.
Metamorphosis is a critical process in the transition from planktonic life to benthic life for marine invertebrates, which is accompanied by a large amount of energy consumption. Previous studies have proved that AMP-activated protein kinase (AMPK), as a vital energy regulator, plays a prominent role in mediating the growth and development of terrestrial animals. However, its function in the growth and development of marine invertebrates, especially in metamorphosis, remains elusive. This study explored the function of AMPK in the larval metamorphosis of . The full-length cDNA of genes in was cloned and characterized, which is composed of three subunits, , , and . Pharmacological tests demonstrated that through the application of an AMPK activator, AMP substantially enhanced the larval metamorphosis rate ( < 0.05). By contrast, the larval metamorphosis rate decreased significantly after being treated with the AMPK inhibitor Compound C ( < 0.05). gene knock-down resulted in a reduction in gene expression ( < 0.05), and the larval metamorphosis of was significantly restrained ( < 0.05). These results indicated that AMPK signaling is vital in the larval metamorphosis of , which advances further understanding in exploring the molecular mechanisms in the metamorphosis of marine invertebrate larvae.
变态是海洋无脊椎动物从浮游生活向底栖生活过渡的关键过程，伴随着大量的能量消耗。先前的研究已经证明，作为一种重要的能量调节剂，AMP 激活蛋白激酶 (AMPK) 在介导陆地动物的生长和发育中起着突出的作用。然而，它在海洋无脊椎动物，特别是变态过程中的功能仍不清楚。本研究探讨了 AMPK 在 幼虫变态中的功能。克隆和鉴定了 的全长 cDNA，它由三个亚基组成， 、 、 。药理试验表明，通过应用 AMPK 激活剂，AMP 显著提高了幼虫变态率（<0.05）。相比之下，用 AMPK 抑制剂 Compound C 处理后，幼虫变态率显著下降（<0.05）。 基因敲低导致 基因表达减少（<0.05）， 幼虫变态明显受到抑制（<0.05）。这些结果表明，AMPK 信号在 幼虫变态中至关重要，这进一步深入了解了海洋无脊椎动物幼虫变态的分子机制。
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