International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Genes (Basel). 2023 Feb 9;14(2):450. doi: 10.3390/genes14020450.
To investigate the regulatory functions of L-arginine and nitric oxide (NO) on metamorphosis, larvae were exposed to an inhibitor of nitric oxide synthase (NOS), aminoguanidine hemisulfate (AGH), and a substrate for NO synthesis, L-arginine. We observed that NO levels showed a significant increase, and this trend continued with L-arginine treatment. When NOS activity was inhibited, the larvae could not synthesize NO, and metamorphosis was not inhibited even in the presence of L-arginine. On transfecting pediveliger larvae with siRNA followed by L-arginine exposure, we found that the larvae did not produce NO and that the larval metamorphosis rate was significantly increased, suggesting that L-arginine regulates larval metamorphosis by promoting NO synthesis. Our findings improve our understanding of the effects of marine environmental factors on larval metamorphosis of mollusks.
为了研究 L-精氨酸和一氧化氮（NO）对变态的调节作用，将幼虫暴露于一氧化氮合酶（NOS）抑制剂氨基胍半硫酸盐（AGH）和 NO 合成的底物 L-精氨酸中。我们观察到 NO 水平显著增加，并且这种趋势随着 L-精氨酸的处理而持续。当抑制 NOS 活性时，幼虫不能合成 NO，即使存在 L-精氨酸，变态也不会被抑制。在用 siRNA 转染足囊幼虫并暴露于 L-精氨酸后，我们发现幼虫没有产生 NO，并且幼虫变态率显著增加，这表明 L-精氨酸通过促进 NO 合成来调节幼虫变态。我们的发现提高了对海洋环境因素对贝类幼虫变态影响的认识。
