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濒危物种 C. Shih 属完整叶绿体基因组序列:特征、物种鉴定和系统发育关系。

Complete Chloroplast Genome Sequence of Endangered Species in the Genus C. Shih: Characterization, Species Identification, and Phylogenetic Relationships.

机构信息

Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences. Peking Union Medical College, Beijing 100193, China.

出版信息

Genes (Basel). 2022 Dec 19;13(12):2410. doi: 10.3390/genes13122410.


DOI:10.3390/genes13122410
PMID:36553677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9778092/
Abstract

C. Shih is a rare genus of the Asteraceae family native to the Taihang Mountains in China. Due to the narrow distribution area, poor reproduction ability and human harvesting, is threatened by extinction. However, the limited genetic information within impede understanding of the conservation efforts and bioprospecting. Therefore, in this study, we reported the complete chloroplast (cp) genome sequences of two species, including and . The cp genomes of and were 151,117 and 151,123 bp, which contained 88 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The repeat sequences, codon usage, RNA-editing sites, and comparative analyses revealed a high degree of conservation between the two species. The 1 gene was identified as a potential molecular marker. The phylogenetic tree demonstrated that was a separate species and not a synonym or variety of The molecular clock showed that two species diverge over a large time span, diverged at 15.24 Mya (Million years ago), whereas diverged at 5.40 Mya We found that and are closely related, which provides new ideas for the development of These results provide biological information and an essential basis to understand the evolutionary history of the species, which will aid in the future the bioprospecting and conservation of endangered species.

摘要

施氏草是菊科的一个珍稀属,原产于中国太行山。由于分布面积狭窄、繁殖能力差和人类采集,该物种受到灭绝的威胁。然而, 内有限的遗传信息阻碍了对保护工作和生物勘探的理解。因此,在本研究中,我们报告了两种 物种,即 和 的完整叶绿体(cp)基因组序列。 和 的 cp 基因组分别为 151,117 和 151,123 bp,包含 88 个蛋白编码基因、37 个 tRNA 基因和 8 个 rRNA 基因。重复序列、密码子使用、RNA 编辑位点和比较分析显示了两个物种之间高度的保守性。我们发现 1 基因是一个潜在的分子标记。系统发育树表明 是一个独立的物种,而不是 的同义词或变种。分子钟表明两个物种在很长的时间跨度内分化, 分化于 15.24 百万年前(Mya),而 分化于 5.40 Mya。我们发现 和 是密切相关的,这为 的开发提供了新的思路。这些结果提供了生物信息和理解 物种进化历史的重要基础,这将有助于未来对濒危物种的生物勘探和保护。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/f20d9b780676/genes-13-02410-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/5c6f15be6166/genes-13-02410-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/23f3ecbcffe1/genes-13-02410-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/e2404ccc7e1d/genes-13-02410-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/c50fbea6a647/genes-13-02410-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/d57af68eb2f0/genes-13-02410-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/b3f4a097c7a2/genes-13-02410-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/e27571d2ed74/genes-13-02410-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/f20d9b780676/genes-13-02410-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/5c6f15be6166/genes-13-02410-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/23f3ecbcffe1/genes-13-02410-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/e2404ccc7e1d/genes-13-02410-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/c50fbea6a647/genes-13-02410-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/d57af68eb2f0/genes-13-02410-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/b3f4a097c7a2/genes-13-02410-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/e27571d2ed74/genes-13-02410-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1167/9778092/f20d9b780676/genes-13-02410-g008.jpg

相似文献

[1]
Complete Chloroplast Genome Sequence of Endangered Species in the Genus C. Shih: Characterization, Species Identification, and Phylogenetic Relationships.

Genes (Basel). 2022-12-19

[2]
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[3]
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[4]
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[6]
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[7]
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[8]
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[9]
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[10]
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引用本文的文献

[1]
Cliff-dwelling plants: rare and precious species in harsh habitats.

Front Plant Sci. 2025-5-5

本文引用的文献

[1]
Localized environmental heterogeneity drives the population differentiation of two endangered and endemic Opisthopappus Shih species.

BMC Ecol Evol. 2021-4-15

[2]
TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data.

Mol Plant. 2020-8-3

[3]
Genetic Divergence and Relationship Among Species Identified by Development of EST-SSR Markers.

Front Genet. 2020-2-28

[4]
PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies.

Mol Ecol Resour. 2019-11-6

[5]
Comparison of Four Complete Chloroplast Genomes of Medicinal and Ornamental Meconopsis Species: Genome Organization and Species Discrimination.

Sci Rep. 2019-7-22

[6]
EST-SSR marker development based on RNA-sequencing of E. sibiricus and its application for phylogenetic relationships analysis of seventeen Elymus species.

BMC Plant Biol. 2019-6-3

[7]
CPGAVAS2, an integrated plastome sequence annotator and analyzer.

Nucleic Acids Res. 2019-7-2

[8]
OrganellarGenomeDRAW (OGDRAW) version 1.3.1: expanded toolkit for the graphical visualization of organellar genomes.

Nucleic Acids Res. 2019-7-2

[9]
Comparative phylogenetic methods and the cultural evolution of medicinal plant use.

Nat Plants. 2018-9-10

[10]
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.

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