传统的等电聚焦不能使免疫复合物解离。

Conventional isoelectric focusing does not dissociate immune complexes.

作者信息

Haas H, Schlaak M

机构信息

Forschungsinstitut Borstel, F.R.G.

出版信息

J Immunol Methods. 1987 Oct 23;103(1):79-85. doi: 10.1016/0022-1759(87)90244-4.

DOI:10.1016/0022-1759(87)90244-4

PMID:3655387

Abstract

Contrary to some reports, isoelectric focusing (IEF) is unable to dissociate immune complexes (IC) into their constituent components. Six model IC systems prepared at various antigen/antibody ratios were readily dissociated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), but not by conventional IEF, independently of several modifications of the running conditions. IC preformed in situ in the IEF gel were not disrupted by subsequent electrophoresis. Moreover, formation of IC was demonstrable in IEF, when applying antigen and antibody preparations in the manner used for counterimmune electrophoresis. Nevertheless, IEF may be of value in the analysis of IC provided the complexes are of small enough size to enter the gel.

摘要

与一些报道相反，等电聚焦（IEF）无法将免疫复合物（IC）解离成其组成成分。以不同抗原/抗体比例制备的六种模型IC系统很容易通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）解离，但传统IEF无法使其解离，这与运行条件的几种修改无关。在IEF凝胶中原位形成的IC不会被随后的电泳破坏。此外，当以用于对流免疫电泳的方式应用抗原和抗体制剂时，在IEF中可证明IC的形成。然而，如果复合物的尺寸足够小以进入凝胶，IEF在IC分析中可能具有价值。