Rates of male pronuclear enlargement in sea urchin zygotes.

Rates of male pronuclear enlargement were determined using synchronous populations of fertilized sea urchin eggs (Arbacia punctulata and Lytechinus variegatus), fixed at regular intervals following insemination and stained with Hoechst 33342. The rate of male pronuclear expansion in both Arbacia and Lytechinus zygotes was linear and significantly affected by polyspermy, temperature, metabolic inhibitors (dinitrophenol, potassium cyanide, sodium azide, antimycin A, and oligomycin), and blockage of cytoplasmic alkalinization. Inhibitors of protein synthesis (emetine and puromycin) and disrupters of cytoskeletal elements (colchicine, nocodazole and cytochalasin B) had no effect on the rate of male pronuclear enlargement.