Department of Biophysics, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jedności 8, 41-200 Sosnowiec, Poland.
Laboratory of Transplant Immunology, Silesian Centre for Heart Diseases, Szpitalna 17, 41-800 Zabrze, Poland.
Medicina (Kaunas). 2022 Nov 30;58(12):1771. doi: 10.3390/medicina58121771.
: Testing for anti-human leukocyte antigen (HLA) antibodies both before and after transplantation is of fundamental significance for the success of lung transplantation. The aim of this study was the evaluation of anti-HLA immunization of patients before and after lung transplant who were subjected to qualification and transplantation. : Prior to the transplantation, patients were examined for the presence of IgG class anti-HLA antibodies (anti-human leukocyte antigen), the so-called panel-reactive antibodies (PRA), using the flow cytometry method. After the transplantation, the class and specificity of anti-HLA antibodies (also IgG) were determined using Luminex. : In the group examined, the PRA results ranged from 0.1% to 66.4%. Low (30%) and average (30-80%) immunization was found in only 9.7% of the group examined. Presence of class I anti-HLA antibodies with MFI (mean fluorescence intensity) greater than 1000 was found in 42.7% of the patients examined, while class II anti-HLA antibodies were found in 38.4%. Immunization levels before and after the transplantation were compared. In 10.87% of patients, DSA antibodies (donor-specific antibodies) with MFI of over 1000 were found. : It seems that it is possible to confirm the correlation between pre- and post-transplantation immunization with the use of the two presented methods of determining IgG class anti-HLA antibodies by increasing the size of the group studied and conducting a long-term observation thereof.
: 在移植前后检测抗人类白细胞抗原（HLA）抗体对于肺移植的成功至关重要。本研究旨在评估接受肺移植资格和移植的患者在移植前后的 HLA 免疫情况。: 在移植前，使用流式细胞术方法检测患者是否存在 IgG 类抗 HLA 抗体（抗人类白细胞抗原），即所谓的 panel-reactive antibodies（PRA）。移植后，使用 Luminex 确定抗 HLA 抗体（也是 IgG）的类别和特异性。: 在检查的组中，PRA 结果范围从 0.1%到 66.4%。仅在检查组的 9.7%中发现低（30%）和中等（30-80%）免疫。在 42.7%的检查患者中发现具有大于 1000 的平均荧光强度（MFI）的 I 类 HLA 抗体，而在 38.4%的患者中发现 II 类 HLA 抗体。比较移植前后的免疫水平。在 10.87%的患者中，发现了具有超过 1000 的 MFI 的 DSA 抗体（供体特异性抗体）。: 似乎可以通过增加研究组的规模并对其进行长期观察，使用两种现有的 IgG 类抗 HLA 抗体检测方法来确认移植前后免疫的相关性。
