Meciano Gabriela de Pauli, Schalch Junior Fernando José, Polizel Guilherme Henrique Gebim, Fernandes Arícia Christofaro, Cracco Roberta Cavalcante, Saran Netto Arlindo, Gomes Rodrigo da Costa, Cônsolo Nara Regina Brandão, Santana Miguel Henrique de Almeida
Department of Animal Science, College of Animal Science and Food Engineering-USP, Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, Brazil.
Embrapa Beef Cattle, Av. Radio Maia, 830, Campo Grande 79106-550, Brazil.
Metabolites. 2022 Dec 10;12(12):1249. doi: 10.3390/metabo12121249.
This study evaluated the effects of gestational supplementation strategy on nitrogen isotopic signature in blood plasma of beef cows and their progeny. The study comprised 15 pregnant Nellore cows divided into three different supplementation protocols: NP) non-programmed group; PP) cows receiving protein−energy supplement in the last third of pregnancy; and FP) cows receiving protein−energy supplement throughout the gestational period. Blood plasma from cows was sampled at the beginning of gestation, in the prepartum, and postpartum periods as well as from their calves at 30 and 180 days of age, for the analysis of stable isotope ratios 15 N/14 N. At pre- and postpartum periods, cows fed PP and FP presented greater abundance of δ15 N compared to NP (p < 0.05) at pre- and postpartum. All three groups showed significant differences (p < 0.05) in the postpartum period. The δ15 N values of calves at 30 days of age differed between the NP group and PP and FP groups (p < 0.05), with no difference (p > 0.05) at 180 days of age. The different gestational supplementation strategies influenced isotopic fractionation of nutrients of cows and their calves after birth, indicating effects on nutritional metabolism and cumulative behavior on isotope abundance related to consumption during gestation.
本研究评估了妊娠期补饲策略对肉牛母牛及其后代血浆中氮同位素特征的影响。该研究包括15头怀孕的内洛尔母牛,分为三种不同的补饲方案:NP)无补饲计划组;PP)在妊娠最后三分之一阶段接受蛋白质 - 能量补充剂的母牛;以及FP)在整个妊娠期接受蛋白质 - 能量补充剂的母牛。在妊娠开始时、产前和产后阶段采集母牛的血浆,以及在其犊牛30日龄和180日龄时采集血浆,用于分析稳定同位素比率15N/14N。在产前和产后阶段,与NP组相比,接受PP和FP补饲的母牛在产前和产后的δ15N丰度更高(p < 0.05)。所有三组在产后阶段均表现出显著差异(p < 0.05)。30日龄犊牛的δ15N值在NP组与PP组和FP组之间存在差异(p < 0.05),而在180日龄时无差异(p > 0.05)。不同的妊娠期补饲策略影响了母牛及其产后犊牛营养物质的同位素分馏,表明对营养代谢以及与妊娠期消耗相关的同位素丰度的累积行为有影响。