Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Recife 50670-901, PE, Brazil.
Departamento de Micologia, Universidade Federal de Pernambuco (UFPE), Recife 50670-901, PE, Brazil.
Molecules. 2022 Dec 14;27(24):8884. doi: 10.3390/molecules27248884.
Yeasts from the complex are clinically relevant due to their high virulence and pathogenicity potential, such as adherence to epithelial cells and emission of filamentous structures, as well as their low susceptibility to antifungals. D-limonene, a natural compound, emerges as a promising alternative with previously described antibacterial, antiparasitic, and antifungal activity; however, its mechanisms of action and antivirulence activity against complex species have not been elucidated. Therefore, in the present study, we aimed to evaluate the antifungal and antivirulence action, as well as the mechanism of action of D-limonene against isolates from this complex. D-limonene exhibited relevant antifungal activity against complex yeasts, as well as excellent antivirulence activity by inhibiting yeast morphogenesis and adherence to the human epithelium. Furthermore, the apoptotic mechanism induced by this compound, which is not induced by oxidative stress, represents an important target for the development of new antifungal drugs.
来自复合体的酵母由于其高毒力和致病性潜力而具有临床相关性，例如对上皮细胞的附着和丝状结构的产生，以及对抗真菌药物的低敏感性。D-柠檬烯作为一种天然化合物，具有先前描述的抗菌、抗寄生虫和抗真菌活性，具有广阔的应用前景；然而，其针对复合体物种的作用机制和抗毒力活性尚未阐明。因此，在本研究中，我们旨在评估 D-柠檬烯对该复合体分离株的抗真菌和抗毒力作用及其作用机制。D-柠檬烯对复合体酵母表现出相关的抗真菌活性，同时通过抑制酵母形态发生和附着在人上皮细胞上，表现出优异的抗毒力活性。此外，该化合物诱导的凋亡机制不是由氧化应激引起的，这代表了开发新型抗真菌药物的一个重要目标。
