Brunner M, Jaswaney V, Wachtel S
Center for Reproductive Biology, Spring Creek Ranch, Collierville, TN 38017.
J Reprod Immunol. 1987 Jul;11(3):181-91. doi: 10.1016/0165-0378(87)90056-8.
Monoclonal H-Y antibody of demonstrated specificity was reacted with soluble H-Y antigen in a newly-developed enzyme-linked immunosorbent assay (ELISA). Typing of cell lines was accomplished by qualitative and quantitative absorption. In one case, the antibody was subdivided into equal portions, each of which was absorbed with 1, 3, 6 or 12 X 10(6) cells from male or female; after absorption, the cells were discarded and the antibody was titrated for residual activity in the ELISA. With increasing cell numbers, optical density scores for male-absorbed antibody approached baseline levels. Although male-female differences were pronounced and highly significant, there was also a fall in reactivity after absorption with female cells.
在一项新开发的酶联免疫吸附测定（ELISA）中，将已证明具有特异性的单克隆H-Y抗体与可溶性H-Y抗原反应。通过定性和定量吸收来完成细胞系的分型。在一个案例中，将抗体分成等量的部分，每部分分别用来自雄性或雌性的1、3、6或12×10⁶个细胞进行吸收；吸收后，丢弃细胞，并在ELISA中滴定抗体的残余活性。随着细胞数量的增加，用雄性细胞吸收后的抗体的光密度得分接近基线水平。尽管雄性与雌性的差异明显且高度显著，但用雌性细胞吸收后反应性也有所下降。
