Mariz Bruno Augusto Linhares Almeida, Sales de Sá Raísa, Araújo Anna Luiza Damaceno, Fernandes Carla Isabelly Rodrigues, Mariano Fernanda Viviane, Santos-Silva Alan Roger, Lopes Marcio Ajudarte, Vargas Pablo Agustin, de Almeida Oslei Paes, Kowalski Luiz Paulo, Jorge Jacks
Oral Diagnosis Department, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, Brazil.
Department of Pathology, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, Brazil.
J Oral Pathol Med. 2023 Feb;52(2):119-126. doi: 10.1111/jop.13398. Epub 2023 Jan 4.
Fibroblast growth factor receptor 1 is a potential prognostic factor for tongue squamous cell carcinoma and is associated with oral epithelial dysplasia grade in oral leukoplakia.
Thirty cases of tongue squamous cell carcinoma and 30 cases of oral leukoplakia were analyzed. Fibroblast growth factor receptor 1 and phosphorylated Akt protein expression were analyzed by immunohistochemistry and quantified using a digital algorithm. Fibroblast growth factor receptor 1 gene amplification was analyzed by fluorescent in situ hybridization in the tongue squamous cell carcinoma cases.
Clinical appearance and dysplasia grade were correlated with oral leukoplakia malignant transformation. Oral leukoplakia cases presenting high fibroblast growth factor receptor 1 expression showed a higher risk of malignant transformation (p = 0.016, HR: 7.3, 95% CI: 1.4-37.4). Phosphorylated Akt showed faint to no expression in oral leukoplakia, which did not correlate with dysplasia grade or malignant transformation. High expression of fibroblast growth factor receptor 1 and phosohorylated Akt were associated with poor overall survival and disease-free survival in tongue squamous cell carcinoma, although only fibroblast growth factor receptor 1 expression was significantly associated with poor overall survival (p = 0.024; HR: 4.9, 95% CI: 1.2-19.9). Cases presenting double fibroblast growth factor receptor 1/phosphorylated Akt overexpression (n = 8) showed markedly impaired overall survival (p = 0.020; HR: 6.4, 95% CI: 1.3-31.1) and disease-free survival (p = 0.001, HR: 13.0, 95% CI: 3.0-55.7). Fibroblast growth factor receptor 1 amplification was observed in 16.6% of tongue squamous cell carcinoma cases, being correlated with vascular and neural invasion (p = 0.001 and 0.017, respectively), but not with fibroblast growth factor receptor 1 protein expression, overall survival, or disease-free survival.
Fibroblast growth factor receptor 1 protein expression is an important prognostic factor in oral leukoplakia and tongue squamous cell carcinoma.
成纤维细胞生长因子受体1是舌鳞状细胞癌的一个潜在预后因素，且与口腔白斑的口腔上皮发育异常分级相关。
分析30例舌鳞状细胞癌和30例口腔白斑病例。通过免疫组织化学分析成纤维细胞生长因子受体1和磷酸化Akt蛋白表达，并使用数字算法进行定量分析。在舌鳞状细胞癌病例中通过荧光原位杂交分析成纤维细胞生长因子受体1基因扩增情况。
临床表现和发育异常分级与口腔白斑恶变相关。成纤维细胞生长因子受体1表达高的口腔白斑病例恶变风险更高（p = 0.016，风险比：7.3，95%置信区间：1.4 - 37.4）。磷酸化Akt在口腔白斑中呈弱阳性至无表达状态，这与发育异常分级或恶变无关。在舌鳞状细胞癌中，成纤维细胞生长因子受体1和磷酸化Akt的高表达与总体生存率和无病生存率低相关，尽管只有成纤维细胞生长因子受体1的表达与总体生存率低显著相关（p = 0.024；风险比：4.9，95%置信区间：1.2 - 19.9）。成纤维细胞生长因子受体1/磷酸化Akt双表达过高的病例（n = 8）总体生存率（p = 0.020；风险比：6.4，95%置信区间：1.3 - 31.1）和无病生存率（p = 0.001，风险比：13.0，95%置信区间：3.0 - 55.7）明显受损。在16.6%的舌鳞状细胞癌病例中观察到成纤维细胞生长因子受体1扩增，其与血管和神经侵犯相关（分别为p = 0.001和0.017），但与成纤维细胞生长因子受体1蛋白表达、总体生存率或无病生存率无关。
成纤维细胞生长因子受体1蛋白表达是口腔白斑和舌鳞状细胞癌的一个重要预后因素。
