National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, Key Laboratory of Parasite and Vector Biology, National Health Commission of People's Republic of China, World Health Organization Collaborating Center for Tropical Diseases, Shanghai 200025, China; Department of Nutrition, Shanghai Fifth People's Hospital, Fudan University, Shanghai 200240, China.
First Affiliated Hospital of Xi'an Medical University, Xian 710077, China.
Acta Trop. 2023 Mar;239:106803. doi: 10.1016/j.actatropica.2022.106803. Epub 2022 Dec 22.
Cystic echinococcosis (CE) is an important zoonotic parasitic disease caused by Echinococcus granulosus (E. granulosus). CE seriously threatens human health and the development of animal husbandry. The Ngari region is one of the world's highest endemic regions for CE, while genetic polymorphisms of E. granulosus were unclear. Paraffin slices of liver Cyst were collected from seventy-nine surgical patients with echinococcosis in the Ngari region. DNA was extracted from samples. The cox1 and cob genes of mitochondrial DNA of E. granulosus were simultaneously amplified and sequenced. The sequencing results were compared with the standard sequence (KU925397.1and HF947574.1). Phylogenetic trees and the haplotype network of cob and cox1 genes were constructed and analyzed genotypes of E. granulosus isolated from humans in the Ngari Region of Tibet. Out of 79 hydatid cyst samples collected from surgery patients, 60 isolates were identified as G1/ G3, and two isolates were identified as G6/ G7. Analysis of the cob/ cox1 genes revealed 9/7 mutations resulting in 8/6 haplotypes, respectively. The cob and cox1 neutrality indices computed by Tajima's D and Fu's Fs tests showed high negative values in Echinococcus granulosus sensu stricto (E. granulosus s. s.). The result suggested that E. granulosus in the Ngari region experienced population expansion or a negative selection. We found that G1/ G3 was still the main genotype, and G6/ G7 was found occasionally in humans of the Ngari region. Therefore, we recommend future surveys and control efforts to investigate G1/ G3 and G6/ G7 transmission in the Ngari region.
泡型包虫病（CE）是一种由细粒棘球绦虫（E.granulosus）引起的重要人畜共患寄生虫病。CE 严重威胁人类健康和畜牧业发展。那曲地区是世界上 CE 流行程度最高的地区之一，而细粒棘球绦虫的遗传多态性尚不清楚。从那曲地区 79 例包虫病手术患者的肝包虫石蜡切片中采集 DNA。同时扩增和测序线粒体 DNA 的 cox1 和 cob 基因。将测序结果与标准序列（KU925397.1 和 HF947574.1）进行比较。构建和分析了来自西藏那曲地区人类的细粒棘球蚴分离株的 cob 和 cox1 基因的系统发育树和单倍型网络。从手术患者的 79 个包虫囊肿样本中采集了 60 个分离株，鉴定为 G1/G3，2 个分离株鉴定为 G6/G7。对 cob/cox1 基因的分析表明，有 9/7 个突变导致 8/6 个单倍型。Tajima's D 和 Fu's Fs 检验计算的 cob/cox1 中性指数表明，在细粒棘球蚴亚种（E.granulosus s.s.）中存在高负值。结果表明，那曲地区的细粒棘球绦虫经历了种群扩张或负选择。我们发现 G1/G3 仍然是主要基因型，G6/G7 偶尔在那曲地区的人群中发现。因此，我们建议未来的调查和控制工作应调查那曲地区 G1/G3 和 G6/G7 的传播。
