紫外线诱导酵母辐射敏感菌株中cyc1等位基因的回复突变。II. rev2突变菌株。
Ultraviolet-induced reversion of cyc1 alleles in radiation sensitive strains of yeast. II. rev2 mutant strains.
作者信息
Lawrence C W, Christensen R B
出版信息
Genetics. 1978 Oct;90(2):213-26. doi: 10.1093/genetics/90.2.213.
Abstract
The range of specificity of the rev2-1 mutation, an allele that reduces the frequency of ochre revertants induced by UV in Saccharomyces cerevisiae (LEMONTT 1971a), has been investigated by examining its influence on the reversion of eleven well-defined and contrasting cyc1 mutations. We have shown, in support of a suggestion of LEMONTT (1971a), that the REV2 gene product is concerned only with the reversion of ochre alleles; it plays virtually no role in the reversion of amber, missense or frameshift mutations. We have also shown that its effect is specific and confined to only some highly revertible ochre alleles. The REV2 gene product appears to enhance reversion at these sites by facilitating the conversion of two otherwise nonmutagenic photo-products into a single premutational lesion. UV-induced killing of rev2-1 strains was found to be significantly greater on fermentable rather than on nonfermentable media.
摘要
rev2-1突变是酿酒酵母中一个能降低紫外线诱导的赭石型回复突变频率的等位基因（LEMONTT，1971a）。通过检测其对11个明确且不同的cyc1突变回复突变的影响，研究了rev2-1突变的特异性范围。我们已证明，支持LEMONTT（1971a）的一个观点，即REV2基因产物仅与赭石型等位基因的回复突变有关；它在琥珀型、错义或移码突变的回复突变中几乎不起作用。我们还表明其作用具有特异性，仅局限于一些高度可回复的赭石型等位基因。REV2基因产物似乎通过促进将两种原本无诱变作用的光产物转化为单一的预突变损伤来增强这些位点的回复突变。发现rev2-1菌株在可发酵培养基上的紫外线诱导杀伤显著大于在非发酵培养基上的。
相似文献
1
Ultraviolet-induced reversion of cyc1 alleles in radiation sensitive strains of yeast. II. rev2 mutant strains.紫外线诱导酵母辐射敏感菌株中cyc1等位基因的回复突变。II. rev2突变菌株。
Genetics. 1978 Oct;90(2):213-26. doi: 10.1093/genetics/90.2.213.
2
Ultraviolet-induced reversion of cyc1 alleles in radiation-sensitive strains of yeast. III. rev3 mutant strains.紫外线诱导酵母辐射敏感菌株中cyc1等位基因的回复突变。III. rev3突变菌株
Genetics. 1979 Jun;92(2):397-408. doi: 10.1093/genetics/92.2.397.
3
UV mutagenesis in radiation-sensitive strains of yeast.酵母辐射敏感菌株中的紫外线诱变
Genetics. 1976 Feb;82(2):207-32. doi: 10.1093/genetics/82.2.207.
4
Genetic analysis of gamma-ray mutagenesis in yeast. I. Reversion in radiation-sensitive strains.酵母中γ射线诱变的遗传分析。I. 辐射敏感菌株中的回复突变
Genetics. 1979 Oct;93(2):361-73. doi: 10.1093/genetics/93.2.361.
5
Ultraviolet-induced reversion of cyc1 alleles in radiation-sensitive strains of yeast. I. rev1 Mutant strains.
J Mol Biol. 1978 Jun 15;122(1):1-21. doi: 10.1016/0022-2836(78)90104-3.
6
The isolation and characterization of ngm2, a mutation that affects nitrosoguanidine mutagenesis in yeast.
Mol Gen Genet. 1986 Jul;204(1):90-7. doi: 10.1007/BF00330193.
7
UV and chemical mutagenesis in rev7 mutants of yeast.酵母rev7突变体中的紫外线和化学诱变
Mol Gen Genet. 1985;200(1):86-91. doi: 10.1007/BF00383317.
8
Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeast.酵母辐射敏感菌株中异-1-细胞色素c赭石突变体紫外线诱导回复突变的特异性和频率
J Mol Biol. 1974 May 5;85(1):137-62. doi: 10.1016/0022-2836(74)90134-x.
9
10
Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae. IV. Influence of DNA replication and excision repair on REV2 dependent UV-mutagenesis and repair.酿酒酵母温度条件突变体中诱变DNA修复的分析。IV. DNA复制和切除修复对REV2依赖性紫外线诱变和修复的影响。
Curr Genet. 1986;10(12):871-8. doi: 10.1007/BF00398283.
引用本文的文献
1
Mutant Gene snm2-1 (ts), conferring thermoconditional mutagen sensitivity in Saccharomyces cerevisiae, is allelic with RAD5.突变基因 snm2-1（ts）使酿酒酵母对热条件下的诱变敏感，与 RAD5 等位。
Curr Genet. 1982 Jul;5(2):93-5. doi: 10.1007/BF00365699.
2
Requirement of Rad5 for DNA polymerase zeta-dependent translesion synthesis in Saccharomyces cerevisiae.酿酒酵母中DNA聚合酶ζ依赖的跨损伤合成对Rad5的需求。
Genetics. 2008 Sep;180(1):73-82. doi: 10.1534/genetics.108.091066. Epub 2008 Aug 30.
3
Mms2-Ubc13-dependent and -independent roles of Rad5 ubiquitin ligase in postreplication repair and translesion DNA synthesis in Saccharomyces cerevisiae.酿酒酵母中Rad5泛素连接酶在复制后修复和跨损伤DNA合成中的Mms2-Ubc13依赖性和非依赖性作用。
Mol Cell Biol. 2006 Oct;26(20):7783-90. doi: 10.1128/MCB.01260-06. Epub 2006 Aug 14.
4
The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination.芽殖酵母RAD6/RAD18 DNA损伤耐受途径的无差错组分采用姐妹染色单体重组。
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15954-9. doi: 10.1073/pnas.0504586102. Epub 2005 Oct 24.
5
Expression of a human cytochrome p450 in yeast permits analysis of pathways for response to and repair of aflatoxin-induced DNA damage.人细胞色素P450在酵母中的表达有助于分析黄曲霉毒素诱导的DNA损伤的应答和修复途径。
Mol Cell Biol. 2005 Jul;25(14):5823-33. doi: 10.1128/MCB.25.14.5823-5833.2005.
6
Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair.酵母MPH1基因在一种无差错的DNA损伤绕过途径中发挥作用，该途径需要同源重组基因，但不需要复制后修复基因。
Genetics. 2004 Apr;166(4):1673-86. doi: 10.1534/genetics.166.4.1673.
7
The srs2 suppressor of UV sensitivity acts specifically on the RAD5- and MMS2-dependent branch of the RAD6 pathway.紫外线敏感性的srs2抑制因子特异性作用于RAD6途径中依赖RAD5和MMS2的分支。
Nucleic Acids Res. 2001 Sep 1;29(17):3487-94. doi: 10.1093/nar/29.17.3487.
8
The RAD5 gene product is involved in the avoidance of non-homologous end-joining of DNA double strand breaks in the yeast Saccharomyces cerevisiae.RAD5基因产物参与酿酒酵母中DNA双链断裂的非同源末端连接的避免过程。
Nucleic Acids Res. 1997 Feb 15;25(4):743-9. doi: 10.1093/nar/25.4.743.
9
The mechanism of untargeted mutagenesis in UV-irradiated yeast.紫外线照射酵母中非靶向诱变的机制。
Mol Gen Genet. 1982;186(1):1-9. doi: 10.1007/BF00422904.
10
Mutagenesis in yeast-misreplication or misrepair?
Mol Gen Genet. 1984;197(3):519-21. doi: 10.1007/BF00329955.
本文引用的文献
1
Mutants of yeast defective in mutation induced by ultraviolet light.在紫外线诱导的突变中存在缺陷的酵母突变体。
Genetics. 1971 May;68(1):21-33. doi: 10.1093/genetics/68.1.21.
2
RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY.酵母的呼吸缺陷型突变体。II. 生物化学
Biochim Biophys Acta. 1964 Jul 15;90:1-15. doi: 10.1016/0304-4165(64)90113-8.
3
Fine-structure mapping and mutational studies of gene controlling yeast cytochrome c1.酵母细胞色素c1基因的精细结构图谱绘制与突变研究
Genetics. 1969 May;62(1):9-22. doi: 10.1093/genetics/62.1.9.
4
Mutagenic specificity: reversion of iso-1-cytochrome c mutants of yeast.
J Mol Biol. 1973 Sep 5;79(1):65-82. doi: 10.1016/0022-2836(73)90270-2.
5
Confirmation of UAG as a nonsense codon in bakers' yeast by amino acid replacements of glutamic acid 71 in iso-1-cytochrome c.通过对异-1-细胞色素c中谷氨酸71进行氨基酸置换，证实UAG是面包酵母中的无义密码子。
J Mol Biol. 1973 Jun 25;78(1):169-84. doi: 10.1016/0022-2836(73)90436-1.
6
UV mutagenesis in radiation-sensitive strains of yeast.酵母辐射敏感菌株中的紫外线诱变
Genetics. 1976 Feb;82(2):207-32. doi: 10.1093/genetics/82.2.207.
7
A deletion map of cyc1 mutants and its correspondence to mutationally altered iso-1-cytochromes c of yeast.酵母cyc1突变体的缺失图谱及其与经突变改变的酵母同工-1-细胞色素c的对应关系。
Genetics. 1975 Sep;81(1):51-73. doi: 10.1093/genetics/81.1.51.
Zotero 插件