Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.
Cholangiocarcinoma Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Am J Trop Med Hyg. 2022 Dec 26;108(2):356-358. doi: 10.4269/ajtmh.22-0478. Print 2023 Feb 1.
Antigen detected in urine for the diagnosis of opisthorchiasis has a low daily variation; however, the longer term variability in antigen concentrations is unknown. In this study, we prospectively monitored Opisthorchis viverrini antigen concentrations for 30 consecutive days and at subsequent monthly intervals in a cohort of opisthorchiasis-positive individuals. On the basis of the monoclonal antibody-based ELISA, the profiles of antigen-positive rate and antigen concentration exhibited no significant change over 30 days with a mean proportion positive of 87.1% (range 73.7%-100%), and the average antigen concentration was 29.7 ± 2.2 ng/mL (mean ± SE). The urine antigen concentration at baseline was similar to the subsequent measurements at 2, 4, 6, and 10 months in the follow-up study (P > 0.05). The consistency and low daily and long-term fluctuation of O. viverrini antigen in urine demonstrates the reliability of urine assay for diagnosis of opisthorchiasis.
在尿液中检测到的用于诊断华支睾吸虫病的抗原每日变异较小；然而，其抗原浓度的长期变异情况尚不清楚。在这项研究中，我们前瞻性地监测了华支睾吸虫阳性个体的连续 30 天和随后的每月间隔的华支睾吸虫抗原浓度。基于基于单克隆抗体的 ELISA，抗原阳性率和抗原浓度的谱在 30 天内没有显著变化，平均阳性比例为 87.1%（范围为 73.7%-100%），平均抗原浓度为 29.7 ± 2.2ng/mL（平均值 ± SE）。在随访研究中，基线时的尿抗原浓度与随后在 2、4、6 和 10 个月时的测量值相似（P＞0.05）。尿液中 O. viverrini 抗原的一致性和低日变异性及长期稳定性表明尿液检测法诊断华支睾吸虫病的可靠性。
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