Shanxi key lab for modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, P.R. China.
Laboratory Animal Center, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
BMC Vet Res. 2022 Dec 26;18(1):453. doi: 10.1186/s12917-022-03531-x.
Zedoary turmeric oil extracted from the roots of curcuma (Curcuma aeruginosa Roxb.) is used for the treatment of myocarditis in China. EMCV infection causes abortion in pregnant sows and myocarditis in piglets. Our previous studies demonstrated that curcumol significantly increased the expression of IFN-β in EMCV infected HEK-293T cells. The present results showed that curcumol inhibits EMCV replication by interfering the host cell cholesterol homeostasis and reducing ROs production through activation of the JAK/STAT signaling pathway.
This study was designed to explore whether curcumol can inhibit the replication of encephalomyocarditis viruses (EMCV) in cell culture. The expression level of JAK1, IRF9, STAT2, P-STAT2, CH25H, PI4KA and OSBP in EMCV-infected HEK-293T cells treated with curcumol, ribavirin or hydroxypropyl-β-CD (HPCD) were determined by Western blotting (WB). The cholesterol level in EMCV infected HEK-293T cells treated with curcumol and HPCD were detected using Amplex™ Red Cholesterol Assay Kit. The antiviral effects of curcumol and HPCD on EMCV were also quantitatively detected by real-time fluorescence quantitative PCR (q-PCR). The amount and morphology of ROs were observed by transmission electron microscopy (TEM).
The results demonstrated that curcumol significantly (P < 0.05) increased the expression of JAK1, IRF9, P-STAT2 and CH25H proteins, while that of STAT2, PI4KA and OSBP were remained unchanged. Compared with virus group (0.134 μg.μg proteins), the total cholesterol level was significantly (P < 0.05) reduced by curcumol (0.108 μg.μg proteins) and HPCD (0.089 μg.μg proteins). Compared with virus group (88237 copies), curcumol (41802 copies) and HPCD (53 copies) significantly (P < 0.05) reduced EMCV load. Curcumol significantly reduced the production of ROs in EMCV-infected HEK-293T cells and activated CH25H through the JAK/STAT signaling pathway.
Curcumol inhibited EMCV replication by affecting the cholesterol homeostasis and the production of ROs in HEK-293T cell.
莪术油是从莪术（Curcuma aeruginosa Roxb.）根部提取的，用于治疗中国的心肌炎。EMCV 感染会导致妊娠母猪流产和仔猪心肌炎。我们之前的研究表明，姜黄素可显著增加 EMCV 感染的 HEK-293T 细胞中 IFN-β的表达。本研究结果表明，姜黄素通过干扰宿主细胞胆固醇稳态并通过激活 JAK/STAT 信号通路减少 ROS 产生，从而抑制 EMCV 复制。
本研究旨在探讨姜黄素是否能抑制细胞培养中的脑心肌炎病毒（EMCV）复制。用姜黄素、利巴韦林或羟丙基-β-环糊精（HPCD）处理 EMCV 感染的 HEK-293T 细胞后，通过 Western blot（WB）测定 JAK1、IRF9、STAT2、P-STAT2、CH25H、PI4KA 和 OSBP 的表达水平。用 Amplex™Red 胆固醇测定试剂盒检测 EMCV 感染的 HEK-293T 细胞中胆固醇水平。用实时荧光定量 PCR（q-PCR）定量检测姜黄素和 HPCD 对 EMCV 的抗病毒作用。用透射电子显微镜（TEM）观察 ROS 的数量和形态。
结果表明，姜黄素显著（P<0.05）增加了 JAK1、IRF9、P-STAT2 和 CH25H 蛋白的表达，而 STAT2、PI4KA 和 OSBP 蛋白的表达则保持不变。与病毒组（0.134μg.μg 蛋白）相比，姜黄素（0.108μg.μg 蛋白）和 HPCD（0.089μg.μg 蛋白）处理后总胆固醇水平显著（P<0.05）降低。与病毒组（88237 拷贝）相比，姜黄素（41802 拷贝）和 HPCD（53 拷贝）显著（P<0.05）降低了 EMCV 载量。姜黄素显著减少 EMCV 感染的 HEK-293T 细胞中 ROS 的产生，并通过 JAK/STAT 信号通路激活 CH25H。
姜黄素通过影响 HEK-293T 细胞中的胆固醇稳态和 ROS 产生来抑制 EMCV 的复制。
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