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通过化学遗传技术激活中脑脚间核可减轻APP/PS1小鼠的学习和记忆障碍。

Activation of the RMTg Nucleus by Chemogenetic Techniques Alleviates the Learning and Memory Impairment in APP/PS1 Mice.

作者信息

Zheng Ling, Wang Zhenjie, Liu Yujia, Zhao Jiapei, Huang Saie

机构信息

Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, People's Republic of China.

College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, People's Republic of China.

出版信息

Neuropsychiatr Dis Treat. 2022 Dec 20;18:2957-2965. doi: 10.2147/NDT.S388832. eCollection 2022.

DOI:10.2147/NDT.S388832
PMID:36573138
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9789721/
Abstract

OBJECTIVE

There is a relationship between non-rapid eye movement (NREM) sleep and Alzheimer's disease (AD). The rostromedial tegmental nucleus (RMTg) is activated can enhance NREM. Therefore, our experiment was designed to investigate the effects of activation of RMTg by chemical genetic techniques on APP/PS1 mice learning and memory.

MATERIALS AND METHODS

After the AAV-hSyn-hM3Dq-mCherry virus was injected into the RMTg nucleus, CNO solution was intraperitoneally injected to activate RMTg. The new object test and Morris water maze were used to determine the learning and memory level; T2-weighted imaging (T2WI) scanning was performed to analyze the volume of hippocampus and entorhinal cortex of each group; The virus transfection status was determined by laser confocal microscope and use immunohistochemical detection to observe the deposition of Beta Amyloid 1-42 (Aβ42).

RESULTS

Activation of RMTg by chemical genetic techniques can reduce the escape latency and increase discrimination index (RI) and the number of crossing platform; Activation of RMTg by chemical genetic techniques reduced the atrophy of the entorhinal cortex. Aβ42 deposition in the brain was decreased after activation of RMTg.

CONCLUSION

Activation of the RMTg nucleus by chemogenetic techniques can improve the learning and memory impairment in APP/PS1 mice.

摘要

目的

非快速眼动（NREM）睡眠与阿尔茨海默病（AD）之间存在关联。被激活的嘴内侧被盖核（RMTg）可增强NREM。因此，我们设计本实验以研究通过化学遗传学技术激活RMTg对APP/PS1小鼠学习和记忆的影响。

材料与方法

将AAV-hSyn-hM3Dq-mCherry病毒注入RMTg核后，腹腔注射CNO溶液以激活RMTg。采用新物体识别试验和莫里斯水迷宫来确定学习和记忆水平；进行T2加权成像（T2WI）扫描以分析每组海马体和内嗅皮质的体积；通过激光共聚焦显微镜确定病毒转染状态，并使用免疫组织化学检测观察β淀粉样蛋白1-42（Aβ42）的沉积情况。

结果

通过化学遗传学技术激活RMTg可缩短逃避潜伏期，增加辨别指数（RI）和穿越平台的次数；通过化学遗传学技术激活RMTg可减轻内嗅皮质的萎缩。激活RMTg后，大脑中Aβ42的沉积减少。

结论

通过化学遗传学技术激活RMTg核可改善APP/PS1小鼠的学习和记忆障碍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/a0555590e56a/NDT-18-2957-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/2bf4cc0f2af6/NDT-18-2957-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/b2420a82014d/NDT-18-2957-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/2cdbadd5e9d4/NDT-18-2957-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/b897d4978ba9/NDT-18-2957-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/68d2eeeae81b/NDT-18-2957-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/a0555590e56a/NDT-18-2957-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/2bf4cc0f2af6/NDT-18-2957-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/b2420a82014d/NDT-18-2957-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/2cdbadd5e9d4/NDT-18-2957-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/b897d4978ba9/NDT-18-2957-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/68d2eeeae81b/NDT-18-2957-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/874a/9789721/a0555590e56a/NDT-18-2957-g0006.jpg

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