Department of Life Sciences, University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet - Cau Giay, Hanoi, Vietnam.
Chem Biodivers. 2023 Jan;20(1):e202200843. doi: 10.1002/cbdv.202200843. Epub 2022 Dec 27.
Golden Camellias have recently been used as a food, cosmetic, and traditional medicine in China and Vietnam. Forty-two species have natural distribution in Vietnam, of which thirty-two species were considered endemic species of this country. The morphology of leaves and flowers of these species were similar; therefore, their taxonomic identification usually needed experts and the authentication has often been confused among species. Our study aims to describe the genetic diversity and the relationship of six species Camellia phanii, Camellia tamdaoensis, Camellia tienii, Camellia flava, Camellia petelotii and Camellia euphlebia by using three chloroplast DNA-barcodes: matK, rbcL and trnH-psbA. We also clarified the significant differences in anatomical characteristics of midvein and blade of their leaves, which suggested the possibility to use these criteria in taxonomy. In addition, preliminary chemical profiles of the methanolic extracts of leaves from six Golden Camellias such as total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC) and chlorogenic acids content (TCGAs) also showed the diversity among them. Interestingly, the discrimination on the catechins profile among six species followed the same tendency with the genetic distance on the phylogeny tree suggesting that catechins (i. e., discriminative catechins) can be biomarkers for the chemotaxonomy of these six Golden Camellias.
金花茶在近期已被广泛应用于食品、化妆品和传统医药领域，在中国和越南均有使用。金花茶在越南共有 42 个天然分布种，其中 32 种被认为是该国特有种。这些物种的叶片和花朵形态相似，因此其分类鉴定通常需要专家进行，且物种之间的鉴定经常混淆。本研究旨在通过三个叶绿体 DNA 条形码（matK、rbcL 和 trnH-psbA）描述六种金花茶（茶 Camellia phanii、越南茶 Camellia tamdaoensis、离蕊金花茶 Camellia tienii、小花金花茶 Camellia flav、显脉金花茶 Camellia petelotii 和小瓣金花茶 Camellia euphlebia）的遗传多样性和种间关系。我们还阐明了它们叶片中主脉和叶片的解剖学特征的显著差异，这表明这些特征在分类学上具有应用的可能性。此外，六种金花茶花叶甲醇提取物的初步化学特征，如总酚含量（TPC）、总黄酮含量（TFC）、总花青素含量（TAC）和绿原酸含量（TCGAs）也显示了它们之间的多样性。有趣的是，六种金花茶之间儿茶素特征的区分与系统发育树中的遗传距离呈相同趋势，表明儿茶素（即具有鉴别能力的儿茶素）可以作为这六种金花茶化学分类学的生物标志物。
