Wyatt J E, Handley P S
Department of Cell and Structural Biology, Medical School, University of Manchester, Great Britain.
Microbios. 1987;51(207):113-23.
Twelve strains of Streptococcus sanguis biotype I and seven strains of Streptococcus sanguis biotype II carrying either peritrichous fibrils or tufts of fibrils, were examined for their susceptibility to aggregation by parotid saliva. Salivary aggregation was evaluated using a spectrophotometric measurement of sedimentation to assess clump size. A clear distinction emerged between structural sub-groups. Irrespective of biotype, strains carrying peritrichous fibrils aggregated strongly whilst tufted strains were little affected. The one strain with peritrichous fimbriae as well as fibrils, was not aggregated by saliva. Pre-treatment of two peritrichously fibrillar strains with parotid saliva reduced their ability to adhere to parotid saliva-coated hydroxyapatite, whereas adhesion of two tufted strains was not inhibited. Inhibition of adhesion may have been due to steric hindrance, but blocking of bacterial adhesins by saliva components could not be discounted.
对12株带有周身纤丝或纤丝簇的血链球菌I型菌株和7株血链球菌II型菌株进行了腮腺唾液聚集敏感性检测。采用分光光度法测量沉降以评估团块大小，从而对唾液聚集进行评估。不同结构亚组之间出现了明显的区别。无论生物型如何，带有周身纤丝的菌株强烈聚集，而有纤丝簇的菌株几乎不受影响。一株既有周身菌毛又有纤丝的菌株未被唾液聚集。用腮腺唾液预处理两株有周身纤丝的菌株会降低它们黏附于腮腺唾液包被的羟基磷灰石的能力，而两株有纤丝簇的菌株的黏附未受抑制。黏附抑制可能是由于空间位阻，但也不能排除唾液成分对细菌黏附素的阻断作用。
