Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Innovation Center of Integration in Production and Education for High-Quality and Safe Livestock and Poultry, Nanchang 330045, China.
Jiangxi Province Key Laboratory of Animal Nutrition, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China; Jiangxi Province Key Innovation Center of Integration in Production and Education for High-Quality and Safe Livestock and Poultry, Nanchang 330045, China.
Poult Sci. 2023 Mar;102(3):102408. doi: 10.1016/j.psj.2022.102408. Epub 2022 Dec 9.
This study tested the hypothesis that glycine improves intestinal barrier function through regulating oxidative stress in broilers exposed to heat stress. A total of 300 twenty-one-day-old female Arbor Acres broilers (600 ± 2.5g) was randomly allocated to 5 treatments (6 replicate of 10 birds each). The 5 treatments were as follows: the control group (CON) was kept under thermoneutral condition (24 ± 1°C) and was fed a basal diet. Broilers fed a basal diet and reared under high ambient temperature (HT) were considered as the HT group (34 ± 1°C for 8 h/d). Broilers fed a basal diet supplemented with 0.5%, 1.0%, and 2.0% glycine and exposed to HT were regarded as the HT + glycine treatments. The results exhibited that heat stress reduced growth performance, serum total antioxidant capacity (T-AOC), and glutathione (GSH) concentration (P < 0.05); increased activity of serum catalase (CAT) and the contents of hydrogen peroxide (HO) and malondialdehyde (MDA) (P < 0.05). HT exposure led to downregulating the mRNA expression of NAD(P)H quinone dehydrogenase 1 (NQO1), Occludin, and zonula occludens-1 (ZO-1) (P < 0.05); enhanced the mRNA levels of Kelch-like ECH-associated protein 1 (Keap1), CAT, glutathione synthetase (GSS), and glutamate-cysteine ligase modifier subunit (GCLM) (P < 0.05); impaired the intestinal morphology (P < 0.05); and altered the diversity and community of gut microbiota (P < 0.05). The final body weight (FBW), ADFI, ADG, and gain-to-feed ratio (G: F) increased linearly or quadratically, and the antioxidant capacity was improved (P < 0.05) with glycine supplementation. Glycine treatment increased the villus height (VH), and villus height to crypt depth ratio (V/C) of the duodenum linearly or quadratically, and linearly increased the VH of jejunum and ileum. The mRNA expression of Occludin, and ZO-1 were increased linearly in the ileum mucosa of broilers subjected to HT. Collectively, these results demonstrated that glycine supplementation alleviates heat stress-induced dysfunction of antioxidant status and intestinal barrier in broilers.
甘氨酸通过调节热应激肉鸡的氧化应激来改善肠道屏障功能。将 300 只 21 日龄雌性 Arbor Acres 肉鸡（600±2.5g）随机分为 5 个处理组（每组 6 个重复，每个重复 10 只鸡）。5 个处理组如下：对照组（CON）在热中性条件下（24±1°C）饲养，并饲喂基础日粮。在高环境温度（HT）下饲养并饲喂基础日粮的肉鸡被视为 HT 组（每天 8 小时，34±1°C）。在 HT 组的基础上添加 0.5%、1.0%和 2.0%甘氨酸的肉鸡被视为 HT+甘氨酸处理组。结果表明，热应激降低了生长性能、血清总抗氧化能力（T-AOC）和谷胱甘肽（GSH）浓度（P<0.05）；增加了血清过氧化氢酶（CAT）活性以及过氧化氢（HO）和丙二醛（MDA）的含量（P<0.05）。HT 暴露导致 NAD(P)H 醌脱氢酶 1（NQO1）、Occludin 和紧密连接蛋白-1（ZO-1）的 mRNA 表达下调（P<0.05）；增强了 Kelch 样 ECH 相关蛋白 1（Keap1）、CAT、谷胱甘肽合成酶（GSS）和谷氨酸半胱氨酸连接酶修饰亚基（GCLM）的 mRNA 水平（P<0.05）；损害了肠道形态（P<0.05）；改变了肠道微生物群的多样性和群落结构（P<0.05）。随着甘氨酸的补充，最终体重（FBW）、采食量（ADFI）、日增重（ADG）和增重饲料比（G：F）呈线性或二次增加，抗氧化能力得到改善（P<0.05）。甘氨酸处理线性或二次增加了十二指肠的绒毛高度（VH）和绒毛高度与隐窝深度比（V/C），并线性增加了空肠和回肠的 VH。HT 处理后，肉鸡回肠黏膜中 Occludin 和 ZO-1 的 mRNA 表达呈线性增加。总之，这些结果表明，甘氨酸的补充缓解了热应激引起的肉鸡抗氧化状态和肠道屏障功能障碍。
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