野生、长尾、抗湿噬菌体在表面扫描磁弹性生物传感器上对鼠伤寒沙门氏菌检测的性能。
Performance of wild, tailed, humidity-robust phage on a surface-scanning magnetoelastic biosensor for Salmonella Typhimurium detection.
机构信息
School of Food Science and Biotechnology, and Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Materials Engineering, and Material Research and Education Center, Auburn University, Auburn, AL 36849, USA.
出版信息
Food Chem. 2023 May 30;409:135239. doi: 10.1016/j.foodchem.2022.135239. Epub 2022 Dec 17.
A wild, tailed phage (TST) was compared with a genetically modified, filamentous phage (FST) for S. Typhimurium (ST) detection. When both phages were introduced into oppositely charged MUA and MUAM sensors, the RU values of TST showed an obvious increase on the MUAM sensor. The sensitivity of TST [54.78 ΔRU/(log PFU/mL)] was greater than that of FST [48.05 ΔRU/(log PFU/mL)]. The binding affinity (K = 1.75 × 10 M) of TST on MUAM sensor was greater than that of FST. Both phages were specific to only ST, and TST exhibited a persistent binding capability at 50 % RH. When each phage-immobilized sensor was employed on chili pepper, the sensitivity [880.80 Hz/(log CFU/mL)] and detection limit (1.31 ± 0.27 log CFU/mL) of TST were significantly greater than those of FST. The orientation of TST on sensor promoted the uniform capture of bacteria and enhanced the reliable performance of a surface-scanning magnetoelastic biosensor.
一种野生长尾噬菌体（TST）与一种基因修饰的丝状噬菌体（FST）在检测鼠伤寒沙门氏菌（ST）方面进行了比较。当两种噬菌体分别引入带相反电荷的 MUAM 和 MUAM 传感器时，TST 的 RU 值在 MUAM 传感器上明显增加。TST 的灵敏度 [54.78 ΔRU/（log PFU/mL）]大于 FST [48.05 ΔRU/（log PFU/mL）]。TST 在 MUAM 传感器上的结合亲和力（K = 1.75×10 M）大于 FST。两种噬菌体均仅对 ST 具有特异性，并且 TST 在 50%相对湿度下表现出持久的结合能力。当每种噬菌体固定传感器用于辣椒时，TST 的灵敏度 [880.80 Hz/（log CFU/mL）]和检测限（1.31±0.27 log CFU/mL）明显大于 FST。TST 在传感器上的取向促进了细菌的均匀捕获，增强了表面扫描磁弹性生物传感器的可靠性能。
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