School of LifeSciences, Zhengzhou University, Zhengzhou, Henan, China.
School of LifeSciences, Zhengzhou University, Zhengzhou, Henan, China; Longhu Laboratory of Advanced Immunology, Zhengzhou, Henan, China.
Biochem Biophys Res Commun. 2023 Feb 5;643:8-15. doi: 10.1016/j.bbrc.2022.12.074. Epub 2022 Dec 24.
Regulators of adult neurogenesis are crucial targets for neuronal repair. Freshwater planarians are ideal model systems for studying neuronal regeneration as they can regenerate their entire central nervous system (CNS) using pluripotent adult stem cells. Here, we identified Djfoxk1 in planarian Dugesia japonica to be required for planarian CNS regeneration. Knockdown of Djfoxk1 inhibits the regeneration of the cephalic ganglia, resulting in the failure of eye regeneration. By RNAi screening of Djfoxk1 downstream genes, we identified Djsnon as another regulator of planarian neuronal regeneration. Inhibition of Djsnon with RNA interference (RNAi) results in similar phenotypes caused by Djfoxk1 RNAi without affecting cell proliferation and wound healing. Our findings show that Djsnon as a downstream gene of Djfoxk1 regulates the regeneration of the planarian CNS.
成体神经发生的调控因子是神经元修复的关键靶点。淡水涡虫是研究神经元再生的理想模型系统，因为它们可以利用多能成体干细胞再生其整个中枢神经系统（CNS）。在这里，我们鉴定了日本三角涡虫中的 Djfoxk1 对于涡虫 CNS 再生是必需的。Djfoxk1 的敲低抑制了头部神经节的再生，导致眼睛再生的失败。通过 Djfoxk1 下游基因的 RNAi 筛选，我们鉴定了 Djsnon 作为另一个调控涡虫神经元再生的因子。用 RNA 干扰（RNAi）抑制 Djsnon 会导致与 Djfoxk1 RNAi 引起的类似表型，而不影响细胞增殖和伤口愈合。我们的研究结果表明，Djsnon 作为 Djfoxk1 的下游基因，调节涡虫 CNS 的再生。
