中性糖鞘脂的质谱分析

Mass Spectrometry of Neutral Glycosphingolipids.

作者信息

Suzuki Akemi, Silsirivanit Atit, Watanabe Takashi, Matsuda Junko, Inamori Kei-Ichiro, Inokuchi Jin-Ichi

机构信息

Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan.

Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

出版信息

Methods Mol Biol. 2023;2613:127-144. doi: 10.1007/978-1-0716-2910-9_11.

DOI:10.1007/978-1-0716-2910-9_11

PMID:36587076

Abstract

This chapter describes the protocols for mass spectrometry (MS) applied to the structural characterization of neutral glycosphingolipids (GSLs) and the determination of neutral GSL contents in biological materials. The structural characterization is performed by thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS) and liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) with reversed phase separation. The content determination is carried out by LC-ESI/MS with multiple reaction monitoring (MRM). These protocols provide clues for the functions of neutral GSLs at the level of a single GSL molecular species.

摘要

本章介绍了应用于中性糖鞘脂（GSLs）结构表征以及生物材料中中性GSL含量测定的质谱（MS）方法。结构表征通过薄层色谱-基质辅助激光解吸电离/质谱（TLC-MALDI/MS）以及采用反相分离的液相色谱-电喷雾电离/质谱（LC-ESI/MS）进行。含量测定通过具有多反应监测（MRM）功能的LC-ESI/MS来完成。这些方法为单个GSL分子种类水平上中性GSL的功能提供了线索。