Suzuki Akemi, Suzuki Minoru, Ito Emi, Nitta Takahiro, Inokuchi Jin-Ichi
Division of Glycopathology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.
School of Integrative and Global Majors, Tsukuba University, Tsukuba, Ibaraki, Japan.
Methods Mol Biol. 2018;1804:207-221. doi: 10.1007/978-1-4939-8552-4_9.
This chapter describes protocols for mass spectrometry (MS) applied to the characterization of ganglioside structures and the determination of ganglioside contents. Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are often used to ionize biological materials and this chapter covers three protocols for atmospheric pressure MALDI MS (AP-MALDI MS), liquid chromatography-ESI MS (LC-ESI MS), and LC-ESI MS with multiple reaction monitoring (MRM). Purified gangliosides were used in AP-MALDI MS analyses while crude preparations of gangliosides were subjected to LC-ESI MS and LC-ESI MS with MRM. The LC protocol includes conditions for both reversed-phase and normal-phase column chromatography.
本章介绍了用于神经节苷脂结构表征和神经节苷脂含量测定的质谱（MS）分析方法。基质辅助激光解吸/电离（MALDI）和电喷雾电离（ESI）常用于生物材料的电离，本章涵盖了三种方法：大气压MALDI质谱（AP-MALDI MS）、液相色谱-ESI质谱（LC-ESI MS）以及带有多反应监测（MRM）的LC-ESI质谱。纯化的神经节苷脂用于AP-MALDI MS分析，而神经节苷脂的粗制品则用于LC-ESI MS和带有MRM的LC-ESI MS分析。液相色谱方法包括反相和正相柱色谱的条件。
