Chen Zhongwen
Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
Methods Mol Biol. 2023;2600:283-289. doi: 10.1007/978-1-0716-2851-5_18.
The ligand-receptor complexes in the cell-cell and cell-ECM interfaces mediate the mechanical coupling of cells to their microenvironment and transduce downstream signals to modulate cell functions. In this chapter I describe a microfabrication strategy to prepare a substrate of spatially segregated supported lipid bilayers and ECM components on which cells can form juxtacrine receptor signaling complexes and integrin adhesions simultaneously. This platform is specifically applicable for microscopically monitoring the signal transduction of each receptor, as well as the modulatory effects of receptor signaling on integrin adhesions and cell behaviors.
细胞-细胞和细胞-细胞外基质界面中的配体-受体复合物介导细胞与其微环境的机械耦合，并转导下游信号以调节细胞功能。在本章中，我描述了一种微制造策略，用于制备具有空间隔离的支持脂质双层和细胞外基质成分的底物，细胞可以在其上同时形成旁分泌受体信号复合物和整合素黏附。该平台特别适用于显微镜监测每个受体的信号转导，以及受体信号对整合素黏附和细胞行为的调节作用。
