通过抑制粘着斑激酶（FAK）磷酸化来测试粘着斑激酶（FAK）在拓扑结构介导的干细胞分化中的作用。
Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK Phosphorylation.
作者信息
Yao Yuan, Yim Evelyn K F
机构信息
Regenerative Nanomedicine Lab, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada.
出版信息
Methods Mol Biol. 2023;2600:297-308. doi: 10.1007/978-1-0716-2851-5_20.
Stem cell differentiation can be modulated by the substrate topographies. Focal adhesion kinase (FAK) has been identified as a key regulator in topography-induced stem cell mechanotransduction. This chapter will describe a protocol to study the effect of FAK phosphorylation inhibition on topography-mediated stem cell differentiation. The FAK phosphorylation was inhibited using a FAK inhibitor and the effects on stem cell differentiation were examined using western blot and immunofluorescence staining.
干细胞分化可受基质拓扑结构的调控。粘着斑激酶（FAK）已被确定为拓扑结构诱导的干细胞机械转导中的关键调节因子。本章将描述一项研究FAK磷酸化抑制对拓扑结构介导的干细胞分化影响的实验方案。使用FAK抑制剂抑制FAK磷酸化，并通过蛋白质免疫印迹和免疫荧光染色检测其对干细胞分化的影响。
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