通过流式细胞术分析肿瘤免疫微环境
Analyzing the Tumor-Immune Microenvironment by Flow Cytometry.
作者信息
Santinon François, Young Yoon Kow, Del Rincón Sonia V, Mann Koren K
机构信息
Lady Davis Institute for Medical Research, McGill University, Montreal, QC, Canada.
Gerald Bronfman Department of Oncology, McGill University, Montreal, QC, Canada.
出版信息
Methods Mol Biol. 2023;2614:17-36. doi: 10.1007/978-1-0716-2914-7_2.
Flow cytometry is an essential tool for studying the tumor-immune microenvironment. It allows us to quickly quantify and identify multiple cell types in a heterogeneous sample. This chapter provides an overview of the flow cytometry instrumentation and a discussion of the appropriate considerations and steps in building a reproducible flow cytometry staining panel. We present an updated lymphoid tissue and solid tumor-infiltrating leucocyte flow cytometry staining protocol and an example of flow cytometry data analysis.
流式细胞术是研究肿瘤免疫微环境的重要工具。它使我们能够快速定量和识别异质样本中的多种细胞类型。本章概述了流式细胞仪设备，并讨论了构建可重复的流式细胞术染色面板时的适当注意事项和步骤。我们展示了一份更新后的淋巴组织和实体瘤浸润白细胞流式细胞术染色方案以及一个流式细胞术数据分析示例。
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