用于高通量药物发现的 3D 水凝胶培养物。
3D Hydrogel Cultures for High-Throughput Drug Discovery.
机构信息
Nemours Biomedical Research, Nemours Children's Hospital - Delaware, Wilmington, DE, USA.
Department of Materials Science and Engineering, University of Delaware, Newark, DE, USA.
出版信息
Methods Mol Biol. 2023;2614:369-381. doi: 10.1007/978-1-0716-2914-7_22.
Our increased understanding of how a cell's microenvironment influences its behavior has fueled an interest in three-dimensional (3D) cell cultures for drug discovery. Particularly, scaffold-based 3D cultures are expected to recapitulate in vivo tissue stiffness and extracellular matrix composition more accurately than standard two-dimensional (2D) monolayer cultures. Here we present a 3D hydrogel cell culture setup suitable for automated screening with standard high-throughput screening (HTS) liquid handling equipment commonly found in a drug discovery laboratory. Further, we describe the steps required to validate the assay system for compound screening.
我们对细胞微环境如何影响其行为的理解不断加深，这激发了人们对用于药物发现的三维（3D）细胞培养的兴趣。特别是，基于支架的 3D 培养有望比标准的二维（2D）单层培养更准确地再现体内组织的刚性和细胞外基质组成。在这里，我们提出了一种 3D 水凝胶细胞培养设置，适用于使用药物发现实验室中常见的标准高通量筛选（HTS）液体处理设备进行自动化筛选。此外，我们还描述了验证化合物筛选测定系统所需的步骤。
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