Perez-Medina Alberto, Galligan James J
Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA.
The Neuroscience Program, Michigan State University, East Lansing, MI, USA.
Adv Exp Med Biol. 2022;1383:33-43. doi: 10.1007/978-3-031-05843-1_4.
ATP is an excitatory and inhibitory neurotransmitter, while nitric oxide (NO) is an inhibitory neurotransmitter in the enteric nervous system (ENS). We used a vesicular nucleotide transporter (SLC17A9, VNUT) antibody and a nitric oxide synthase (NOS) antibody to identify purinergic and nitrergic nerves in mouse and guinea ileum. Mouse: VNUT-immunoreactivity (ir) was detected in nerve fibers in myenteric ganglia and circular muscle. VNUT-ir fibers surrounded choline acetyltransferase (ChAT), nitric oxide synthase (nNOS), and calretinin-ir neurons. VNUT-ir nerve cell bodies were not detected. Tyrosine hydroxylase (TH)-ir nerves were detected in myenteric ganglia and the tertiary plexus. Guinea pig: VNUT-ir was detected in neurons and nerves fibers and did not overlap with NOS-ir nerve fibers. VNUT-ir was detected in nerve fibers in ganglia but not nerve cell bodies. VNUT-ir nerve fibers surrounded NOS-ir and NOS neurons. NOS-ir and VNUT-ir nerve fibers did not overlap in myenteric ganglia or circular muscle. VNUT-ir nerves surrounded some ChAT-ir neurons. VNUT-ir and ChAT-ir were detected in separate nerves in the CM. VNUT-ir nerve fibers surrounded calretinin-ir neurons.Conclusions: VNUT-ir neurons likely mediate purinergic signaling in small intestinal myenteric ganglia and circular muscle. ATP and NO are likely released from different inhibitory motorneurons. VNUT-ir and ChAT-ir interneurons mediate cholinergic and purinergic synaptic transmission in the myenteric plexus.
三磷酸腺苷（ATP）是一种兴奋性和抑制性神经递质，而一氧化氮（NO）是肠神经系统（ENS）中的一种抑制性神经递质。我们使用囊泡核苷酸转运体（SLC17A9，VNUT）抗体和一氧化氮合酶（NOS）抗体来鉴定小鼠和豚鼠回肠中的嘌呤能神经和氮能神经。小鼠：在肌间神经节和环行肌的神经纤维中检测到VNUT免疫反应性（ir）。VNUT-ir纤维围绕着胆碱乙酰转移酶（ChAT）、一氧化氮合酶（nNOS）和钙视网膜蛋白-ir神经元。未检测到VNUT-ir神经细胞体。在肌间神经节和三级神经丛中检测到酪氨酸羟化酶（TH）-ir神经。豚鼠：在神经元和神经纤维中检测到VNUT-ir，且与NOS-ir神经纤维不重叠。在神经节的神经纤维中检测到VNUT-ir，但未在神经细胞体中检测到。VNUT-ir神经纤维围绕着NOS-ir和NOS神经元。在肌间神经节或环行肌中，NOS-ir和VNUT-ir神经纤维不重叠。VNUT-ir神经围绕着一些ChAT-ir神经元。在环行肌中，VNUT-ir和ChAT-ir存在于不同的神经中。VNUT-ir神经纤维围绕着钙视网膜蛋白-ir神经元。结论：VNUT-ir神经元可能在小肠肌间神经节和环行肌中介导嘌呤能信号传递。ATP和NO可能从不同的抑制性运动神经元释放。VNUT-ir和ChAT-ir中间神经元在肌间神经丛中介导胆碱能和嘌呤能突触传递。
