Department of Dermatology, Mayo Clinic, Rochester, Minnesota, USA.
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.
Exp Dermatol. 2023 Apr;32(4):502-510. doi: 10.1111/exd.14738. Epub 2023 Jan 10.
Oral lichen planus (OLP) confers an approximately 1% risk of transformation to oral squamous cell carcinoma (OSCC). Early identification of high-risk OLP would be very helpful for optimal patient management. We aimed to discover specific tissue-based protein biomarkers in patients with OLP who developed OSCC compared to those who did not. We used laser capture microdissection- and nanoLC-tandem mass spectrometry to assess protein expression in fixed lesional mucosal specimens in patients with indolent OLP (no OSCC after at least 5-year follow-up, n = 6), transforming OLP (non-dysplastic epithelium with lichenoid inflammation marginal to OSCC, n = 6) or normal oral mucosa (NOM, n = 5). Transforming OLP protein profile was enriched for actin cytoskeleton, mitochondrial dysfunction and oxidative phosphorylation pathways. CA1, TNNT3, SYNM and MB were overexpressed, and FBLN1 was underexpressed in transforming OLP compared with indolent OLP. Integrin signalling and antigen presentation pathways were enriched in both indolent and transforming OLP compared with NOM. This proteomic study provides potential biomarkers, such as CA1 overexpression, for higher-risk OLP. While further validation studies are needed, we propose that epithelial-mesenchymal transition may be involved in OLP carcinogenesis.
口腔扁平苔藓（OLP）发生口腔鳞状细胞癌（OSCC）的风险约为 1%。早期识别高危 OLP 对患者的最佳管理非常有帮助。我们旨在发现与未发生 OSCC 的 OLP 患者相比，发生 OSCC 的 OLP 患者中特定的组织蛋白生物标志物。我们使用激光捕获显微切割和纳升 LC-串联质谱法评估了惰性 OLP（至少 5 年随访后无 OSCC，n=6）、转化 OLP（非异型上皮伴边缘 OSCC 的苔藓样炎症，n=6）或正常口腔黏膜（NOM，n=5）患者病变黏膜标本中的蛋白质表达。转化 OLP 的蛋白图谱富含肌动蛋白细胞骨架、线粒体功能障碍和氧化磷酸化途径。与惰性 OLP 相比，转化 OLP 中 CA1、TNNT3、SYNM 和 MB 过表达，FBLN1 表达下调。整合素信号和抗原呈递途径在惰性和转化 OLP 中均比 NOM 丰富。这项蛋白质组学研究为高风险 OLP 提供了潜在的生物标志物，如 CA1 过表达。虽然还需要进一步的验证研究，但我们提出上皮-间充质转化可能参与 OLP 的癌变。
