甲硫氨酸脑啡肽作用的突触前位点在小鼠脊髓培养物中得到证实。

A presynaptic locus of the action of Met-enkephalin demonstrated in mouse spinal cord cultures.

作者信息

Jia M, Nelson P G

机构信息

Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Peptides. 1987 May-Jun;8(3):565-8. doi: 10.1016/0196-9781(87)90024-6.

DOI:10.1016/0196-9781(87)90024-6

PMID:3658816

Abstract

Monosynaptic excitatory post-synaptic potentials (EPSPs) evoked in spinal cord (SC) neurons by stimulation of dorsal root ganglion (DRG) neurons in cell cultures were reduced by perfusion application of the opiate peptide, Met-enkephalin (2-4 microM). In about 2/3 of cases examined, EPSPs evoked by stimulation of spinal cord cells were also reduced by Met-enkephalin. The effects were antagonized by concomitant perfusion with naloxone (1-2 microM) and recovered when perfusion with Met-enkephalin was stopped. Statistical analysis of synaptic responses indicated that the reduction of EPSP amplitude was due, at least to a major extent, to a decrease in presynaptic transmitter release.

摘要

在细胞培养中，通过刺激背根神经节（DRG）神经元诱发脊髓（SC）神经元产生的单突触兴奋性突触后电位（EPSP），在灌注阿片肽甲硫氨酸脑啡肽（2 - 4 microM）后降低。在约2/3的检测病例中，刺激脊髓细胞诱发的EPSP也被甲硫氨酸脑啡肽降低。这些效应可被同时灌注纳洛酮（1 - 2 microM）所拮抗，当停止灌注甲硫氨酸脑啡肽时效应恢复。对突触反应的统计分析表明，EPSP幅度的降低至少在很大程度上是由于突触前递质释放减少所致。