影响人供体角膜上皮完整性的因素。

Factors affecting the epithelial integrity of human donor corneas.

机构信息

Cornea and Anterior Segment Service, L. V. Prasad Eye Institute, Bhubaneswar, Odisha, India.

Department of Ophthalmic Biophysics, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.

出版信息

Indian J Ophthalmol. 2023 Jan;71(1):91-94. doi: 10.4103/ijo.IJO_1470_22.

DOI:10.4103/ijo.IJO_1470_22

PMID:36588215

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10155530/

Abstract

PURPOSE

To evaluate various factors affecting the integrity of human donor corneal epithelium.

METHODS

Donor corneal buttons were evaluated for epithelial defect (ED) and exposure. The slit-lamp photographs were taken on day 01, and the data such as age and gender of the donor, cause of death, refrigeration of cadavers, death-to-preservation time (DPT), experience of technician, and distance from site of collection to eye bank were collected.

RESULTS

A total of 100 consecutive corneal buttons belonging to 56 donors were evaluated. The median age of donors was 50 years. Males constituted 45 (80.4%). The mean DPT was 9.7 ± 5.3 hours. After death, 34 donors (60.7%) were refrigerated before the collection/retrieval. Most of the corneas (80%) were recovered by technicians having an experience of 0-5 years. Thirty-one donors (55.3%) were located at 1-50 km from the eye bank. The mean area of exposure was 15 ± 4.3 mm. The mean area of ED was 28.7 ± 5.9 mm. ED was significantly associated with refrigeration of cadavers and longer DPT. On multivariate analysis, only DPT was found to be significantly associated (P = 0.006; odds ratio [OR] = 1.54 ± 0.24) with the presence of ED. After transplantation, only two corneas had persistent epithelial defects and were treated successfully using various interventions.

CONCLUSION

Integrity of donor corneal epithelium is mainly influenced by the refrigeration of cadavers and DPT.

摘要

目的

评估影响人供体角膜上皮完整性的各种因素。

方法

评估供体角膜瓣的上皮缺损（ED）和暴露情况。在第 01 天拍摄裂隙灯照片，并收集供体的年龄和性别、死亡原因、尸体冷藏、死亡至保存时间（DPT）、技术员经验以及采集点到眼库的距离等数据。

结果

共评估了 100 个连续的角膜瓣，属于 56 位供体。供体的中位年龄为 50 岁。男性占 45 例（80.4%）。平均 DPT 为 9.7±5.3 小时。死后，34 例（60.7%）在采集/检索前进行了冷藏。大多数角膜（80%）由经验在 0-5 年的技术员回收。31 例供体（55.3%）距离眼库 1-50 公里。暴露面积平均为 15±4.3mm。ED 的平均面积为 28.7±5.9mm。ED 与尸体冷藏和较长的 DPT 显著相关。多变量分析显示，仅 DPT 与 ED 的存在显著相关（P=0.006；优势比[OR]为 1.54±0.24）。移植后，仅有两例角膜出现持续性上皮缺损，并通过各种干预措施成功治疗。