Taguchi Satoru, Choudhury Mohammed E, Mikami Kanta, Utsunomiya Ryo, Yano Hajime, Tanaka Junya
Department of Molecular and Cellular Physiology, Graduate School of Medicine, Ehime University, Toon, Japan.
Ann Rehabil Med. 2022 Dec;46(6):320-328. doi: 10.5535/arm.22105. Epub 2022 Dec 31.
To determine the appropriate time points to start regular exercise which could reduce age-related anxiety and impaired social behavior.
For this study, 8-week-old male Wistar rats were divided into three groups: no exercise (NoEX), short-term exercise (S-Ex), and long-term exercise (L-Ex) groups. S-Ex-group rats started treadmill exercise at 12 months of age, while L-Ex rats started from at 2 months of age. Exercise rats were forced to walk on the treadmill three times per week, with 1- to 2-day intervals for 10 minutes during the first 2 weeks, at 10 m/min until 17 months of age, and at 8 m/min thereafter. At 19 months of age, behavioral tests were performed to assess the effects of exercise on age-induced behavioral change as well as quantitative polymerase chain reaction were done to uncover the mechanism behind the behavioral changes.
Anxiety-like behavior was improved by long-term exercise. Additionally, rats belonging to the S-Ex and L-Ex groups showed improved social behavior and increased curiosity about interesting objects. The qPCR data showed that treadmill exercise suppressed the expression of immediate-early genes in the prefrontal cortex of the aged rats.
This study suggests that long-term exercise represses early response genes, and in this way, it increases resistance to stress, diminishes anxiety-related behavior, and improves social behavior. These findings underscore the need to consider appropriate time to start exercise to prevent stress induced anxiety related behavior.
确定开始规律运动的合适时间点，以减少与年龄相关的焦虑和社交行为受损。
在本研究中，8周龄雄性Wistar大鼠被分为三组：不运动组（NoEX）、短期运动组（S-Ex）和长期运动组（L-Ex）。S-Ex组大鼠在12月龄开始跑步机运动，而L-Ex组大鼠从2月龄开始。运动组大鼠每周被迫在跑步机上行走三次，前两周每隔1至2天运动10分钟，速度为10米/分钟，直至17月龄，此后速度为8米/分钟。在19月龄时，进行行为测试以评估运动对年龄诱导的行为变化的影响，并进行定量聚合酶链反应以揭示行为变化背后的机制。
长期运动改善了焦虑样行为。此外，S-Ex组和L-Ex组的大鼠表现出社交行为改善，对有趣物体的好奇心增加。qPCR数据表明，跑步机运动抑制了老年大鼠前额叶皮层中即早基因的表达。
本研究表明，长期运动可抑制早期反应基因，从而增加对压力的抵抗力，减少焦虑相关行为，并改善社交行为。这些发现强调了需要考虑开始运动的合适时间，以预防压力诱导的焦虑相关行为。
