一种从羊水细胞培养物中制备前中期染色体的简单方法。

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures.

作者信息

Cheung S W, Crane J P, Johnson A, Simms L, Reid J

机构信息

Department of Obstetrics and Gynecology, Washington University School of Medicine, Jewish Hospital of St. Louis, Missouri 63110.

出版信息

Prenat Diagn. 1987 Jul;7(6):383-8. doi: 10.1002/pd.1970070602.

DOI:10.1002/pd.1970070602

PMID:3658911

Abstract

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin-EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up to 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.

摘要

本文描述了一种从羊水细胞培养物中制备前中期染色体的简单方法。该技术基于几个关键步骤，包括：（1）降低秋水仙酰胺浓度，（2）减少胰蛋白酶-乙二胺四乙酸（EDTA）处理时间，以及（3）通过适当调整细胞浓度使细胞保持单悬浮状态。通常可以制备出单倍体组带分辨率高达800条带的染色体。所描述的方法在定义和确定细微结构畸变的临床意义方面特别有用。