Petrova Valentina A, Dubashynskaya Natallia V, Gofman Iosif V, Golovkin Alexey S, Mishanin Alexander I, Aquino Arthur D, Mukhametdinova Daria V, Nikolaeva Alexandra L, Ivan'kova Elena M, Baranchikov Alexander E, Yakimansky Alexander V, Ivanov Vladimir K, Skorik Yury A
Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi VO 31, St. Petersburg 199004, Russian Federation.
Almazov National Medical Research Centre, Akkuratova 2, St. Petersburg 197341, Russian Federation.
Int J Biol Macromol. 2023 Feb 28;229:329-343. doi: 10.1016/j.ijbiomac.2022.12.305. Epub 2022 Dec 30.
Polymeric nanocomposite materials have great potential in the development of tissue-engineered scaffolds because they affect the structure and properties of polymeric materials and regulate cell proliferation and differentiation. In this work, cerium oxide nanoparticles (CeONPs) were incorporated into a chitosan (CS) film to improve the proliferation of multipotent mesenchymal stem cells (MSCs). The citrate-stabilized CeONPs with a negative ζ-potential (-25.0 mV) were precoated with CS to obtain positively charged particles (+20.3 mV) and to prevent their aggregation in the composite solution. The composite CS-CeONP films were prepared in the salt and basic forms using a dry-cast process. The films obtained in both forms were characterized by a uniform distribution of CeONPs. The incorporation of CeONPs into the salt form of CS increased the stiffness of the CS-CeONP film, while the subsequent conversion of the film to the basic form resulted in a decrease in both the Young's modulus and the yield stress. The redox activity (Ce ⇌ Ce) of cerium oxide in the CS-CeONP film was confirmed by thermal oxidative degradation. In vitro culture of MSCs showed that the CS-CeONP film has good biocompatibility, and in vivo experiments demonstrated its substantial regenerative potential.
聚合物纳米复合材料在组织工程支架的开发中具有巨大潜力，因为它们会影响聚合物材料的结构和性能，并调节细胞增殖和分化。在这项工作中，将氧化铈纳米颗粒（CeONPs）掺入壳聚糖（CS）膜中，以促进多能间充质干细胞（MSCs）的增殖。将具有负ζ电位（-25.0 mV）的柠檬酸盐稳定的CeONPs预先用CS包覆，以获得带正电的颗粒（+20.3 mV），并防止它们在复合溶液中聚集。采用干铸法制备了盐形式和碱形式的CS-CeONP复合膜。两种形式获得的膜均具有CeONPs分布均匀的特征。将CeONPs掺入CS的盐形式中会增加CS-CeONP膜的硬度，而随后将膜转化为碱形式会导致杨氏模量和屈服应力均降低。通过热氧化降解证实了CS-CeONP膜中氧化铈的氧化还原活性（Ce ⇌ Ce）。MSCs的体外培养表明CS-CeONP膜具有良好的生物相容性，体内实验证明了其巨大的再生潜力。
