Tohmi Manavu, Cang Jianhua
Department of Biology, University of Virginia, Charlottesville, VA 22904, USA.
Department of Psychology, University of Virginia, Charlottesville, VA 22904, USA.
iScience. 2022 Dec 9;26(1):105778. doi: 10.1016/j.isci.2022.105778. eCollection 2023 Jan 20.
Despite its importance, the development of higher visual areas (HVAs) at the cellular resolution remains largely unknown. Here, we conducted 2-photon calcium imaging of mouse HVAs lateromedial (LM) and anterolateral (AL) and V1 to observe developmental changes in visual response properties. HVA neurons showed selectivity for orientations and directions similar to V1 neurons at eye opening, which became sharper in the following weeks. Neurons in all areas over all developmental stages tended to respond selectively to dots moving along an axis perpendicular to their preferred orientation at slow speeds, suggesting a certain level of conventional motion coding already at eye opening. In contrast, at high speeds, many neurons responded to dots moving along the axis parallel to the preferred orientation in older animals but rarely after eye opening, indicating a lack of motion-streak coding in the earlier stage. Together, our results uncover the development of visual properties in HVAs.
尽管其很重要，但在细胞分辨率水平上高等视觉区域（HVAs）的发育仍很大程度上未知。在此，我们对小鼠的高等视觉区域的内外侧（LM）和前外侧（AL）以及初级视觉皮层（V1）进行了双光子钙成像，以观察视觉反应特性的发育变化。高等视觉区域神经元在睁眼时对方向的选择性与初级视觉皮层神经元相似，在接下来的几周内变得更加明显。在所有发育阶段，所有区域的神经元在低速时倾向于对沿垂直于其偏好方向的轴移动的点有选择性地做出反应，这表明在睁眼时就已经有一定水平的传统运动编码。相比之下，在高速时，许多年长动物的神经元对沿平行于偏好方向的轴移动的点有反应，但在睁眼后很少有这种情况，这表明早期阶段缺乏运动条纹编码。总之，我们的结果揭示了高等视觉区域视觉特性的发育情况。
