Garrido-Jareño Marta, Sahuquillo-Arce José Miguel, Rodríguez-Vega Héctor, Lloret-Sos Carmen, Gil-Brusola Ana, López-Hontangas José Luis, Nuñez-Beltran María, Tortosa-Carreres Jordi, García-García José Ángel, Cordón Lourdes, Puchades-Carrasco Leonor, de Santivañes Carmen Carreras-Gil, Pineda-Lucena Antonio, Pemán-García Javier
Drug Discovery Unit, Health Research Institute Hospital La Fe, Valencia, Spain.
Microbiology Department, University and Polytechnic Hospital La Fe, Avenida Fernando Abril Martorell, 106 Torre A 3ª Planta, 46026, Valencia, Spain.
Med Microbiol Immunol. 2023 Feb;212(1):93-102. doi: 10.1007/s00430-022-00759-0. Epub 2023 Jan 3.
Measurement of anti-pneumococcal capsular polysaccharides (anti-PnPs) IgG titers is an important tool in the immunologic assessment of patients with suspected immunodeficiency disorders (ID) to reduce the morbi-mortality and minimize severe infections. Retrospectively, we studied the relationship among anti-PnPs IgG response to 3 doses of Prevenar®13, levels of immune system components, leukocyte populations, and clinical data in children with ID. Serum samples were collected at least 4 weeks post vaccination. Subsequently, multi-serotype enzyme-linked immunosorbent assay (ELISA) was performed. Eighty-seven children (under 12 years) were enrolled. Primary immunodeficiency disorder (PID) was the most common disorder (45) followed by possible immunodeficiency disorder (POID) (19), secondary immunodeficiency disorder (SID) (15), and mixed immunodeficiency disorder (MID) (8). The median age was 3 (1.50-5.33) years, 65% of patients were male. Deficient production of anti-PnPs IgG (titer ≤ 50 mg/L) was detected in 47 patients (54%), especially in the MID group, all of them under immunosuppressive therapy. In PCV13 responders, the mean of leukocyte population levels was higher with statistically significance differences in CD4 + /CD8 + T lymphocytes (p = 0.372, p = 0.014) and CD56 + /CD16 + NK (p = 0.016). Patients with previous bone marrow transplantation were the worst PCV13 responders. Pneumococcal IgG antibody titers (post-vaccination) along with clinical and analytical markers represented.
测量抗肺炎球菌荚膜多糖（抗PnPs）IgG滴度是对疑似免疫缺陷疾病（ID）患者进行免疫评估的重要工具，以降低发病死亡率并使严重感染最小化。我们回顾性研究了ID患儿中抗PnPs IgG对3剂沛儿13（Prevenar®13）的反应、免疫系统成分水平、白细胞群体与临床数据之间的关系。在接种疫苗后至少4周采集血清样本。随后，进行多血清型酶联免疫吸附测定（ELISA）。共纳入87名12岁以下儿童。原发性免疫缺陷疾病（PID）是最常见的疾病（45例），其次是可能的免疫缺陷疾病（POID）（19例）、继发性免疫缺陷疾病（SID）（15例）和混合免疫缺陷疾病（MID）（8例）。中位年龄为3（1.50 - 5.33）岁，65%的患者为男性。47例患者（54%）检测到抗PnPs IgG产生不足（滴度≤50 mg/L），尤其是在MID组，他们均接受免疫抑制治疗。在PCV13应答者中，白细胞群体水平的平均值较高，CD4 + /CD8 + T淋巴细胞（p = 0.372，p = 0.014）和CD56 + /CD16 + NK（p = 0.016）存在统计学显著差异。既往有骨髓移植史的患者是最差的PCV13应答者。呈现了肺炎球菌IgG抗体滴度（接种疫苗后）以及临床和分析标志物。
