比较转录组分析揭示了甜玉米采后时间和货架期调控的分子机制。

Comparative transcriptome analyses shed light on the regulation of harvest duration and shelf life in sweet corn.

机构信息

Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, Zhejiang 322100, China.

出版信息

Food Res Int. 2023 Jan;163:112188. doi: 10.1016/j.foodres.2022.112188. Epub 2022 Nov 20.

DOI:10.1016/j.foodres.2022.112188

PMID:36596131

Abstract

Harvest duration and postharvest shelf life are two of the most important characters for sweet corn. However, the regulatory mechanism remains unclear. We performed a comparative transcriptome analysis of long harvest-duration and shelf-life sweet corn (LHS) and short harvest-duration and shelf-life field corn (SHS) at three stages, i.e. 10 days after pollination (10DAP), 22 days after pollination (22DAP), and 7 days after harvest (7DAH). We have observed the major transcriptome changes accompanying the harvest process in LHS corn. Gene expression pattern analysis and differentially expressed genes (DEGs) functional enrichments suggested an association between ROS metabolism in kernels with harvest duration and postharvest shelf life. The genes encoding cytochrome P450, peroxidase, peroxiredoxin, glutathione peroxidase, and glutathione S-transferase were upregulated specifically in LHS kernels during and after harvest compared to SHS kernels. These novel findings reveal a new regulatory mechanism of corn post-harvest shelf life and should be useful for extending harvest duration and shelf life for sweet corn.

摘要

采后货架期是甜玉米最重要的两个特性之一。然而，其调控机制尚不清楚。我们对长采后货架期和长货架期甜玉米（LHS）和短采后货架期和短货架期田间玉米（SHS）在授粉后 10 天（10DAP）、授粉后 22 天（22DAP）和收获后 7 天（7DAH）三个阶段进行了比较转录组分析。我们观察到 LHS 玉米在采后过程中伴随的主要转录组变化。基因表达模式分析和差异表达基因（DEGs）功能富集表明，ROS 代谢与收获时间和货架期之间存在关联。与 SHS 玉米相比，LHS 玉米在收获期间和收获后，细胞色素 P450、过氧化物酶、过氧化物还原酶、谷胱甘肽过氧化物酶和谷胱甘肽 S-转移酶的编码基因特异性地上调。这些新发现揭示了玉米采后货架期的新调控机制，这对于延长甜玉米的收获时间和货架期应该是有用的。