Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510222, China.
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province 510222, China.
Food Res Int. 2023 Jan;163:112268. doi: 10.1016/j.foodres.2022.112268. Epub 2022 Nov 29.
The present study aimed to investigate the effects of five chitooligosaccharide monomers of different molecular weights on immunomodulatory activity in macrophage-like RAW264.7 cells. The incubation of various chitooligosaccharide monomers enhanced phagocytosis and pinocytosis activity toward Staphylococcus aureus and Escherichia coli in RAW264.7 cells. The incorporation of chitooligosaccharide monomers significantly boosted the generation of reactive oxygen species and reactive nitrogen species, as well as the release of inflammatory cytokines. To further explore the mechanism of inflammation regulated by chitooligosaccharide, the activation inhibitors of NF-кB (CAPE) and TLR-4 (TAK-242) were utilized, the determination data demonstrated that chitobiose suppressed the expression of inflammatory cytokines and NF-кB p65. In addition, the investigation results revealed that the presence of the mannose receptor inhibitor (mannan) suppressed chitohexaose-induced phagocytic activity and inflammatory cytokines. These results suggested that the five distinct chitooligosaccharide monomers had inconsistent effects, the chitobiose and chitohexaose exhibiting the best biological activity in activating RAW264.7 cells, promoting cell proliferation, and increasing non-specific immunity.
本研究旨在探讨五种不同分子量的壳寡糖单体对巨噬细胞样 RAW264.7 细胞免疫调节活性的影响。不同壳寡糖单体的孵育增强了 RAW264.7 细胞对金黄色葡萄球菌和大肠杆菌的吞噬和胞饮作用。壳寡糖单体的加入显著促进了活性氧和活性氮的产生，以及炎症细胞因子的释放。为了进一步探讨壳寡糖调节炎症的机制，利用 NF-κB（CAPE）和 TLR-4（TAK-242）的激活抑制剂进行了测定，结果表明，壳二糖抑制了炎症细胞因子和 NF-κB p65 的表达。此外，研究结果表明，甘露糖受体抑制剂（甘露聚糖）抑制了壳六糖诱导的吞噬活性和炎症细胞因子。这些结果表明，五种不同的壳寡糖单体具有不同的作用，其中壳二糖和壳六糖在激活 RAW264.7 细胞、促进细胞增殖和增强非特异性免疫方面表现出最佳的生物学活性。
