Filipczak Nina, Li Xiang, Saawant Gaurav Rajan, Yalamarty Satya Siva Kishan, Luther Ed, Torchilin Vladimir P
Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA 02115, USA.
Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA 02115, USA; State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Chinese Medicine, Nanchang, China.
J Control Release. 2023 Feb;354:109-119. doi: 10.1016/j.jconrel.2022.12.062. Epub 2023 Jan 5.
Neutrophil extracellular traps (NETs) are structures consisting of decondensed chromatin with associated proteins, including histones and antimicrobial peptides, released from activated neutrophils. They are believed to be one of the body's first lines of defense against infectious agents. Despite their beneficial effect on the immune response process, some studies indicate that their excessive formation and the associated accumulation of extracellular DNA (eDNA) together with other polyelectrolytes (F-actin) plays an important role in the pathogenesis of many diseases. Thus NETs formation and removal are clinically significant. The monoclonal antibody 2C5 has strong specificity for intact nucleohistones (NS) and targets NS in NETs as we previously confirmed. Creation of a nano preparation that can specifically recognize and destroy NETs represents the aim for treatment many diseases. 2C5 antibody functionalized micelles coated with DNase I were created to achieve this aim.
中性粒细胞胞外诱捕网（NETs）是由解聚的染色质与相关蛋白质组成的结构，这些蛋白质包括组蛋白和抗菌肽，由活化的中性粒细胞释放。它们被认为是机体抵御感染因子的第一道防线之一。尽管它们对免疫反应过程有有益作用，但一些研究表明，它们的过度形成以及细胞外DNA（eDNA）与其他聚电解质（F-肌动蛋白）的相关积累在许多疾病的发病机制中起重要作用。因此，NETs的形成和清除具有临床意义。单克隆抗体2C5对完整的核组蛋白（NS）具有很强的特异性，正如我们之前所证实的，它靶向NETs中的NS。创建一种能够特异性识别和破坏NETs的纳米制剂是治疗许多疾病的目标。为实现这一目标，制备了用脱氧核糖核酸酶I包被的2C5抗体功能化胶束。
